Objective To study dectin-1 mRNA expression in lung of different immune status mice infected with Aspergillusfumigatus, and to explore the influence of immunosuppressive agent on the expression of dectin-1 and its relationship with the progression of disease. Methods Mice were divided into four groups which were normal control, immuncompromised, immuncompromised with A.fumigatus inoculation and immuncompetent with A.fumigatus inoculation groups. We explored the kinetic mRNA expression of dectin-1 in lung of different groups by real-time quantitative PCR. Pulmonary fungal burden assessment was performed to reflect the progressing of disease during the experimental time course. Results On day 3 after inoculation, pulmonary fungal burden of the immuncompromised mice was higher than that of the immuncompetent group. On day 1 and 3, dectin-I mRNA expression in lung of the immuncompromised group was much lower than that of the normal control. On day 3, dectin-1 mRNA expression in lung of the immuncompetent mice infected with A.fumigatus was much higher than that of the immuncompromised with A.fumigatus inoculation and the normal control groups ( P < 0.05 ). Conclusion During the infection, expression of dectin-1 in lung of the immuncompetent group was strikingly increased, which may play an important role on the defence to A.fumigatus invasion. Cyclophosphamide inhibited the expression of dectin-1 in lung of mice which may be one of the mechanisms of the invasive pulmonary aspergillosis development induced by eyclophosphamide.%目的 研究不同免疫状态小鼠感染烟曲霉后肺组织dectin-1 mRNA表达变化.探讨免疫抑制剂对dectin-1表达的影响与疾病发展的相互关系.方法 小鼠分4组:正常对照组、免疫抑制组、免疫抑制种菌组及免疫正常种菌组.采用实时荧光定量PCR法检测各组小鼠不同时间点肺组织dectin-1 mRNA表达.同时进行肺组织真菌载量测定观察疾病发展情况.结果 小鼠感染烟曲霉后第3天,免疫抑制种菌组菌载量明显高于正常种菌组.在感染后第1天及第3天,免疫抑制组小鼠肺组织dectin-1表达较正常对照组明显降低;第3天,免疫正常种菌组表达较正常对照组及免疫抑制种菌组明显升高(P<0.05).结论 对于免疫正常宿主,dectin-1可能在防御烟曲霉感染中起重要作用.环磷酰胺可降低肺组织dectin-1 mRNA表达,可能与其促发侵袭性肺曲霉病有关.
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