减肥后与健康相关的脂肪因子在动物模型中的表达

摘要

Objective Some studies have been shown that reduced weight can increase serum anti-inflammatory cyto-kines and reduced pro-inflammatory cytokines, but whether the change of weight loss can alter mRNA expression of these adipokines are still unknown. Therefore, the research will investigate that after weight loss, the mRNA expres-sion of health-related adipokines in adipose tissue in obesity animal model. Method 12 LDLr (-/-) KO mice were induced obesity firstly and then 8 of them were subdivided into two subgroups, fed with high fat diet to keep obesity state or with low fat diet to lost weight for 82 days. After 82 days, mRNA expression of seven adipokines would be analyzed. Result There were no significant changes between HF×82 group and LF×82 group for seven adipokines mRNA expression. Conclusion These findings indicate that after weight loss by diet, health-related adipokines ex-pression in an animal model of obesity does not change.%目的：一些研究已经指出减低体重可以增加血液中抗炎因子的分泌并减少促炎因子,但是体重的减轻是否能改变这些脂肪因子mRNA的表达是未知的。因此本实验将会研究在减肥后,在肥胖小鼠脂肪组织里,与健康相关的脂肪因子的mRNA的表达情况。方法12只LDLr (-/-)敲除的老鼠将会先诱导为肥胖,然后其中8只分为两组,一组持续高脂肪喂养,另一组改为低脂肪喂养以达到减轻体重的目的。82天之后,7个脂肪因子的mRNA表达将会被测量。结果7个脂肪因子的表达在减肥组( HF ×82)和肥胖组(LF×82)中并没有显著的区别。结论结论通过饮食诱导减轻体重之后,与健康相关的脂肪因子mRNA表达在动物模型中并没有改变。