目的:观察喂养不耐受对极低出生体重儿肠道菌群的影响.方法:选择30例喂养不耐受的住院极低出生体重儿为观察组,同时选择30例喂养耐受的极低出生体重儿为对照组,于生后2d、出现喂养不耐受后2d和喂养不耐受消失后5d分别留取患儿大便标本,采用实时荧光定量PCR技术检测标本中的肠道乳酸杆菌及双歧杆菌.结果:喂养不耐受组中乳酸杆菌的数量分别为[(4.15 ±0.25) cfu/g,(5.39 ±0.53) cfu/g,(7.32 ±0.80) cfu/g],对照组分别为[(4.17±0.27)cfu/g,(7.84±0.35) cfu/g,(7.61 ±1.35) cfu/g],双歧杆菌的数量分别为[(4.35±0.23) cfu/g,(5.01 ±0.48) cfu/g,(7.12±1.22) cfu/g],对照组分别为[(4.37 ±0.22) cfu/g,(8.52±0.23) cfu/g,(7.40±1.33) cfu/g].两组于出现喂养不耐受后2d,两种细菌数量比较差异具有统计学意义(P＜0.05),而喂养不耐受出现之前和消失后5d两组细菌数量比较差异无统计学意义(P＞0.05).结论:喂养不耐受对极低出生体重儿肠道菌群的变化有明显影响.喂养不耐受使肠道双歧杆菌和乳酸杆菌数量降低.%Objective:To study the impact of feeding intolerance on intestinal flora of very low birth weight infants.Methods:Study group including 30 very low birth weight infants of feeding intolerance,and 30 feeding tolerance preterm infants were control group.At two days after birth,after clinical manifestations of feeding intolerance appeared 2 d,after the recovery of feeding intolerance 5 d,using realtine PCR technology to measure intestinal specimens lactobacillus and bifidobacterium.Results:At three periods,numbers of intestinal lactobacilli in feeding intolerance group were [(4.15 ±0.25)cfu/g,(5.39 ±0.53) cfu/g,(7.32 ±0.80) cftt/g];in the control group were [(4.17 ±0.27) cfu/g,(7.84 ±0.35) cfu/g,(7.61 ± 1.35) cfu/g].Numbers of intestinal bifidobacteria were [(4.35 ±0.23) cfu/ g,(5.01 ± 0.48) cfu/g,(7.12 ± 1.22) cfu/g];in the control group were [(4.37 ± 0.22) cfu/g,(8.52 ± 0.23) cfu/g,(7.40 ± 1.33) cfu/g].There was statistically significant difference between the feeding intolerance group and the control group when feeding intolerance occurred 2 d(P ＜ 0.05).There was not statistically significant difference between the feeding intolerance group and the control group before the appearance of tolerance and after the recovery of feeding intolerance 5 d(P ＞ 0.05).Conclusion:Feeding intolerance is an important factor affecting intestinal microbiota in very low birth weight infants.The numbers of intestinal specimens lactobacillus and bifidobacterium were significantly lower.
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