天冬氨酸修饰纳米羟基磷灰石/聚乳酸复合物制备软骨组织工程支架及其生物性能研究

摘要

目的:通过天冬氨酸修饰的纳米羟基磷灰石(Asp-nHA)和左旋聚乳酸(PLLA)复合,制备软骨组织工程支架,并进行体外实验研究,探索其作为软骨组织工程支架的可行性.方法:以碳二亚胺盐酸盐/N-羟基琥珀酰亚胺偶联法制备Asp-nHA,并与PLLA复合,获得新型Asp-nHA/PLLA纳米复合支架.比较成骨细胞在该新型材料表面的粘附、生长和增殖的情况.结果:加入nHA后,Asp-nHA/PLLA复合材料可显著增强细胞的粘附、生长、增殖;Asp-nHA/PLLA的生物学性能明显优于PLLA及HA/PLLA,且Asp-nHA/PLLA具有最强的体外诱导成骨细胞分化能力.结论:Asp-nHA/PLLA制备简单,具有良好的生物相容性和成骨活性,是一种性能良好的骨组织工程支架材料.%Objective To design and prepare surface modified cartilage tissue scaffolds with the use of aspartic acid-modified nano-hydroxyapatite (Asp-nHA) and poly L-lactic acid (PLLA),and evaluate its feasibility as a scaffold for cartilage tissue engineering through in vitro experiment.Methods Asp-nHA was synthesized with the standard EDC/NHS coupling method,and the resultant Asp-nHA was conjugated to PLLA to form Asp-nHA/PLLA composite scaffold.The osteoblast adhesion,expansion and proliferation on Asp-nHA/PLLA composite scaffold were evaluated.Results Osteoblast adhesion,expansion and proliferation on Asp-nHA/PLLA composite scaffold were significantly increased due to the addition ofnHA.The in vitro analysis illustrated that Asp-nHA/PLLA composite was superior to PLLA and HA/PLLA in bio-characterizations and had the strongest ability to induce osteogenic differentiation in vitro.Conclusion Asp-nHA/PLLA is simple to be prepared and its good biocompatibility and osteogenic activity make it become a good kind of scaffold for cartilage tissue engineering.