Objective To prepare Fe3O4-loaded lipid perfluorooctylbromide (Fe3O4-PFOB) nanoparticles , and to explore its potential application as a M RI contrast agent . Methods Fe3O4 -PFOB nanopartic.les were prepared using a film -ultrasonic method . Then the physical and chemical properties were observed . Twenty-four SD rats were randomized into 3 groups (each n= 8) : group 1 (injection with Fe3O4-PFOB nanoparticles ) , group 2 (injection with PFOB nanoparticles ) and group 3 (injection with normal saline ) . MR scanning were performed 24 h after the administration . Immediately after MR scanning , sections of the rats 'Iivers were stained with HE and Prussian blue . Results Fe3 01 -PFOB nanoparticles consisted of a shell of' lecithin loaded with Fe3O4 particles and a liquid core of PFOB . Fe3O4 -PFOB nanoparticles were spherical in shape and homogeneous in size (272. 9±19. 5 )nm . The signal intensity of liver parenchyma reduced markedly 24 h after injection of' Fe3O4-PFOB nanoparticles . The signal ratio of liver to muscle decreased signif'icantly compared with the other groups .Prussian blue stain showed accumulation of blue particles within liver tissues in group 1 , while no particles were f'ound in the other groups . HE stain showed no change of liver f'ormation in all groups . Conclusion The newly f'ormulated Fe3O4PFOB nanoparticles can eff'ectively enhance M R imaging in vivo , and has a potential to be a versatile image probe for M RI .%目的 制备载Fe3O4液态氟碳(Fe3O4-PFOB)纳米粒,并探讨其增强磁共振成像效果.方法 采用薄膜-超声法制备Fe3O4-PFOB纳米粒,并检测其理化性质.将24只健康SD大鼠随机分为3组:Fe3O4-PFOB纳米粒组(静脉注射Fe3O4-PFOB纳米粒)、PFOB纳米粒组(静脉注射PFOB纳米粒)及生理盐水组(静脉注射生理盐水).对各组大鼠于处理后24 h行肝脏MR扫描.扫描完成后取出大鼠肝脏组织,进行HE及普鲁士蓝染色观察.结果 所得样品为膜壳装载Fe3O4、中心包裹液态氟碳的纳米粒,呈球形,粒径(272.9±19.5)nm.注射Fe3O4-PFOB纳米粒24 h后,大鼠肝实质信号显著降低,肝脏-肌肉信号比明显减少,与其余两组比较差异均有统计学意义.普鲁士蓝染色可见Fe3O4-PFOB纳米粒组大鼠肝实质内有较多蓝色颗粒分布,其余两组染色阴性.HE染色各组大鼠肝脏均未见明显的形态学改变.结论 Fe3O4-PFOB纳米粒能有效增强磁共振成像效果,可以用作MRI阴性对比剂.
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