骨髓间充质干细胞抑制Wistar大鼠脊髓损伤区神经元凋亡的实验研究

摘要

目的 探讨骨髓间充质干细胞(BMSCs)是否具有抑制神经元凋亡的作用.方法 Wistar大鼠120只随机平均分为4组:对照1组为空白对照,对照2组、两治疗组均采用改良Allen法建立大鼠下胸段脊髓损伤模型.治疗1组、治疗2组分别经尾静脉和脊髓损伤区注入BMSCs.术后用免疫组化检测各组Caspase-3、Bax、Bcl-2的表达.结果 术后7天,治疗2组bax、caspase-3阳性细胞表达少于对照2组(P＜0.05),治疗1组Bcl-2阳性细胞表达多于对照2组(P＜0.05);移植后15天,治疗1组bax以及治疗2组caspase-3阳性细胞表达少于对照2组(P＜0.05),治疗1组及治疗2组Bcl-2阳性细胞表达多于对照2组(P＜0.05);移植后30天,治疗1组bax阳性细胞表达少于对照2组(P＜0.05),治疗1组、治疗2组Bcl-2阳性细胞表达多于对照2组(P＜0.05).结论 BMSCs移植上调Bcl-2蛋白表达,下调Caspase-3、Bax蛋白表达,具有抗凋亡作用,可减轻脊髓继发性损伤.%Objective To approach bone m esenchym al stem cells(BMSCs) whether or not would inhibit the Neuronal apoptosis. Methods To divided 120 W istar rats into 4 groups randamly .The control group 1 is a blank control, the control group 2 and two treatment groups was produced to spinal cord injury model by modified Allen's crack method .Two treatment groups' BM SC s which were isolated culture in vitro inside the model Ihrough the vena caudalis and the partial injury randamly .After transplantation ,use the method of immunohistochemical staining detect each groups' Caspase-3 ,Bax,Bcl-2 expression. Results At the 7th day after trans-plantation,The Bxa,caspase-3 positive cells expressed in the treatment group 2 less than the control group 2 (P＜0 .05),The Bcl-2 positive cells of the treatment group 1 expressed more than the control group 2 ( P＜0 .05 ) ;At the 15th day after tansplantation ,the Bxa positive cells of the treatment group 1 and the caspase-3 positive cells of the treatment group 2 expressed less than the control group 2 ( P＜0 .05 ) ,the Bcl-2 positive cells of the treatment group 1 and the treatment group 2 expressed more than the control group 2 ( P＜0 .05) ; At the 30th day after transplantation ,the Bxa positive cells of the treatment group 1 expressed less than the control group 2 (P ＜0 .05 ) ,the Bcl-2 positive cells of the treatment group 1 and the treatment group 2 expressed more than the surgery group (P＜0 .05) .Conclusion BM SCs transplantation could increase the Bcl-2 protein's expression,reduce Caspase-3 and Bax protein's expression ,have the role of anti-apoptosis,which could reduce spinal cord secondary injury.