激活素-卵泡抑素系统在病理性瘢痕中的表达

摘要

目的 探讨激活素-卵泡抑素系统(activin-FS)在病理性瘢痕组织中的表达.方法 收集2014-06/2017-06吉林铭医整型美容医院整形外科组织标本,分为病理性瘢痕组46例(包括瘢痕疙瘩组25例和增生性瘢痕组21例)、非病理性瘢痕组30例和正常皮肤组27例.采用免疫组化(Immunohistochemistry,IHC)方法分别检测activin A及FS的蛋白表达情况;RNA酶保护试验(RNase protection assay,RPA)分析activin A的mRNA表达水平.结果 免疫组化结果显示,activin A和FS在正常皮肤和病理性瘢痕的表皮基底层均有表达,且病理性瘢痕组较正常皮肤组染色程度加深(P<0.05).RNA酶保护试验结果显示,正常皮肤组和非病理性疤痕组activin-A mRNA表达水平极低,而病理性瘢痕组表达则明显增多.结论 activin-FS系统可能参与病理性瘢痕的发生发展.%Objective The aim of this study is to detect the expressions of activin-A and follistatin (FS)and investi-gate their relationship in pathogenesis and development of pathological scars.Methods Experimental samples were ob-tained from the patients,who underwent burn and plastic surgery at Department of Plastic Surgery,Jilin Moii Medical Cosmetology Hospital,from June 2014 to June 2017,including 25 patients with keloid,and 21 patients with hypertroph-ic scars.Normal skin and non-pathological scars from additional 27 and 30 cases respectively were served as controls. The expression of activin-A and FS protein in pathological scars,non-pathological scars and normal skin were examined by immunohistochemistry (IHC)(P < 0.05).The expression of mRNA in these tissues were performed by RNAase protection assay (RPA).Results IHC showed increased localization of both activin-A and FS in the basal layer of epi-dermis of pathological scars compared with normal tissue.Increased mRNA expression for activin A was observed in pathological scars by performing RPA.Conclusion These findings emphasize the importance of the activin-FS system in biology and pathogenesis of pathological scars.