Objective To assess the curative effect of injection of doxorubicin-carrying liquid-solid phase variant in situ gel for residual rabbit VX2 liver cancer after high intensity focused ultrasound (HIFU) irradiation. Methods VX2 liver tumor models were set up in 24 rabbits, and non-complete HIFU ablation was performed. The rabbits were randomly divided into 2 groups (each n = 12), i. e. HIFU + DOX-ISFI group (injecting doxorubicin-carrying liquid-solid phase variant in situ gel after HIFU irradiation) and HIFU+N-ISFI group (injecting drug-free liquid-solid phase variant in situ gel after HIFU irradiation). Tumor growth rate and PCNA expression were compared between the two groups. The distribution of drug in cancer was observed with a fluorescent imager of frozen tissue samples. Results Tumor growth rate of HIFU+DOX-ISFI group was obviously lower than that of HIFU + N-ISFI group (P<0. 05). Proliferating index of HIFU+DOX-ISFI group was dramatically lower than that of HIFU+N-ISFI group (P<0. 05). Fluorescence imaging showed the drug distributed from the injection center to the surrounding area, like a ladder shape. Conclusion Injection of doxorubicin-carrying liquid-solid phase variant in-situ gel can effectively treat the residual rabbit VX2 liver cancer after HIFU treatment.%目的 观察载阿霉素液-固相变型原位注射凝胶(DOX-ISFI)治疗高强度聚焦超声(HIFU)消融兔VX2肝癌后残癌的疗效.方法 以24只兔建立VX2肝癌模型,对肿瘤行HIFU不全消融,随机分为HIFU消融与DOX-ISFI联合治疗组(HIFU+DOX ISFI组)、HIFU消融与空白液-固相变原位注射凝胶联合治疗组(HIFU+N-ISFI组),每组12只,比较两组肿瘤生长率、PCNA表达情况,并以冰冻切片荧光显像观测药物瘤内分布.结果 处理后HIFU+ DOX-ISFI组肿瘤生长明显减慢,其生长率明显低于HIFU+ N-ISFI组(P＜0.05)；HIFU+DOX-ISFI组肿瘤增殖指数明显低于HIFU+N-ISFI组(P＜0.05)；荧光显像显示药物从注射中心向周围呈阶梯状分布.结论 DOX-ISFI能有效治疗HIFU消融兔VX2肝癌后的残癌.
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