促红细胞生成素对神经胶质细胞增殖、凋亡、迁移能力的影响

摘要

目的 探讨促红细胞生成素(EPO)对神经胶质细胞增殖、凋亡、迁移能力的影响,为EPO神经保护作用机制的研究提供依据.方法 体外培养用不同浓度EPO混合培养基培养U251细胞,用噻唑蓝(MTT)法筛选EPO的最佳浓度,以EPO最佳浓度的细胞为实验组,未加EPO细胞为阴性对照组,通过细胞克隆实验检测细胞增殖水平变化、通过划痕实验检测细胞迁移能力变化、通过transwell实验检测细胞侵袭能力变化、通过细胞流式术观察EPO对神经胶质细胞凋亡能力的变化.结果 通过MTT实验检测EPO最佳浓度为300 IU/L;克隆实验检测发现细胞增殖水平增加;划痕实验检测发现细胞迁移能力增加;Transwell实验检测发现细胞侵袭能力增加;细胞流式术发现细胞的凋亡能力降低.结论 本实验得出EPO可促进U251细胞的增殖、迁移、侵袭,并抑制其凋亡,因此证实EPO对神经细胞具有保护作用,在临床上为新生儿脑损伤的早期干预治疗提供一条新途径和理论根据.%Objective To investigate the effects of erythropoietin(EPO)on the activity of proliferation,apoptosis and migration of glio-ma U251 cells. Methods The glioma U251 cells were cultured in vitro and optimal concentration of EPO was screened with MTT. Then glioma U251 cells were divided into negative control group and the experimental group(EPO optimum concentration for the ex-perimental group).The proliferation,migration,invasion and apoptosis capability of glioma U251 cells were detected by cloning exper-iments,scratch test,transwell experiment and the cells flow cytometry.Results The result of MTT was that the optimal concentration of EPO was 300 IU/mL.The cloning experiment showed that the level of cell proliferation was increased.The result of the scratch test showed that cell migration was increased.Transwell experiment showed the capacity of cell invasion was increased.The cell flow cy-tometry showed that the ability of apoptosis was reduced.Conclusion The experimentally derived EPO could promote the prolifera-tion,migration and invasion,and inhibit the apoptosis of glioma U251 cells.It could be seen the EPO had a protective effect on nerve cells. It provided a new way and theoretical basis for the intervention in the early of neonatal brain injury in clinical.