抗纤灵方抑制肾络病慢性肾衰竭大鼠TGF-β／P13 K／Akt信号旁路的实验研究

摘要

Objective:Replicate 5/6 nephrectomy CRF rat’s models to observe the effects on TGF-β/P13K/Akt signal passage of experimental rats with“Kang Xian Ling Decoction”. Methods:Divide 36 rats into three groups( normal group,model group with physiological saline,treatment group with the drug );then replicate 5/6 nephrectomy CRF rat ’s models except normal group for 2 months;apply medical technology such as biochemical mensuration of blood and organization,Radioimmunoassay and Western blot to detect renal function and 24hUPro,wrenal pathology and TGF-β/P13K/Akt signal passage. Results:After experi-ment,compared with model group,serum creatinineresults of mode group dramatically reduced(P﹤0. 01),serum creatinine of treat-ment group increase obviously(P﹤0. 05),Pathology observation MASSON;compared with model group,Hypertrophy on glomerulus and basilar membrane on capillary vessel of treatment group has weakened. There has been an obvious decrease in mesentery stroma;collagenous fibre elements is lower than model group;During western blot examination,compared with model group,gray value of p-Akt in treatment group decline obviously. Conclusion:Chinese medicine“Kang Xian Ling Decoction”can significantly improve kid-ney function of 5/6 nephrectomy CRF rats through reducing albuminuria and serum creatinine to protect renal function with inhibiting TGF-β/P13K/Akt signal passage.%目的：采用5／6肾切除方法建立慢性肾衰竭大鼠模型，观察抗纤灵对5／6肾切除大鼠肾组织中TGF-β／PI3k／Akt信号通路的影响。方法：将SD大鼠分为正常组、模型组（生理盐水）、治疗组（抗纤灵）各12只，除正常组外均采用5／6肾切除方法建立慢性肾衰竭大鼠模型，疗程2个月。应用生化、放射免疫法、western blot等技术，检测肾功能、24 h尿蛋白定量、肾脏病理，检测TGF-β／P13K／Akt信号通路蛋白表达。结果：实验结束时，与模型组相比较，抗纤灵组的血肌酐数值显著下降（ P﹤0．01）、蛋白尿下降差异有统计学意义（ P﹤0．05）；病理MASSON染色：抗纤灵组肾小球肥大减弱，毛细血管基底膜增厚减轻，系膜基质明显减少，胶原纤维成分低于模型组；western blot检测，与模型组相比，抗纤灵组的p-Akt灰度比值显著下降。结论：抗纤灵方可能通过抑制TGF-β／PI3K／Akt信号通路，从而有效地降低5／6肾切除大鼠的血肌酐，减轻肾组织纤维化、改善肾功能、降低蛋白尿。