虎藻脂肝消胶囊对非酒精性脂肪性肝病大鼠脂质过氧化及胰岛素抵抗的影响

摘要

目的:观察虎藻脂肝消胶囊对非酒精性脂肪性肝病(NAFLD)大鼠血清脂质过氧化、胰岛素抵抗的影响,探讨其治疗NAFLD的作用机制.方法:采用高脂乳剂灌胃并配合自由饮用蔗糖水建立脂肪肝胰岛素抵抗SD大鼠模型.造模后,以虎藻脂肝消胶囊高、中、低剂量混悬液分别灌胃大鼠,并以多烯磷脂酰胆碱灌胃大鼠为阳性对照,均持续6周.检测大鼠血清超氧化物歧化酶(SOD)、丙二醛(MDA)、葡萄糖(FBG)、胰岛素(FINS)的水平,并采用HOMA法计算胰岛素抵抗指数(HOMA-IR).结果:中、高剂量组大鼠SOD显著高于模型对照组(P＜0.01、P＜0.01);而低、高剂量组大鼠的MDA与模型对照组相比显著降低(P ＜0.05、P＜0.01).低剂量治疗组大鼠FBG较模型对照组显著性降低(P＜0.05),其余各组与模型对照组比较无统计学意义;高剂量组大鼠FINS与模型对照组比较显著降低(P＜0.01);低、高剂量组大鼠HOMA-IR与模型对照组比较显著降低(P＜0.01).结论:虎藻脂肝消胶囊能显著提高NAFLD大鼠SOD活性,降低氧自由基代谢产物MDA的含量,且对大鼠胰岛素抵抗表现出了较好的治疗作用.%Objective: To study the effect of capsule Huzao Zhiganxiao on SOD, MDA, FBG, FINS and HOMA-IR in rat model of nonalcoholic fatty liver disease (NAFLD) , and unveil its mechanism of antagonizing NAFLD. Methods; The model of NAFLD were made by intragastric administration with high fat emulsion and free drink of cane suger syrup. SOD, MDA, FBG, FINS were detected, HOMA-IR was calculated. Results: The activity of SOD was higher and the content of MDA was lower in control group than in model group that control group. The control group had descendant effect on HOMA-IR. Conclusion-. Capsule Huzao Zhiganxiao can improve SOD activity, reduce oxygen free metabolism related to the content of MDA and improve the insulin resistance of rats.