丹参对暴发性肝衰竭大鼠肝损伤及肝再生的影响

摘要

Objective: To study the effect of Radix Salviae Miltiorrhizae on liver damage and liver regeneration in thioacet-amide-induced acute hepatic failure in rats.Methods: The rats were randomly divided into normal group , model group , Radix Salviae Miltiorrhizae group and lyophilized bovine transfer factor control group.Medications were given 1ml/100g respectively 3 days before hypodermically injected with thioacetamide ( TAA ) once a day.The rats were hypodermically injected with TAA 600mg· kg-1 twice at same time for two days to induce the model of acute hepatic failure rat except the normal group injected with the 0.9%salt solution hypodermically.Forty-eight hours later , the blood were collected to measure ALT , AST, TBil, and at the same time, the parameters of liver regeneration were observed as following: liver mitotic index and proliferation cell nucler antigen.Results: Radix Salviae Miltiorrhizae could obviously decrease ALT , AST, TBil, improved liver mitotic index and pro-liferation cell nucier antigen of rats with acute hepatic failure.Comparing with model group and control group , there were signifi-cant difference (P<0.05 or P<0.01) .Conclusion: Radix Salviae Miltiorrhizae has esistance against the rat with acute he-patic failure , improve liver regeneration.%目的：探讨丹参对暴发性肝衰竭（ FHF）大鼠肝损伤及肝再生的影响。方法： Wistar大鼠随机分为4组：正常对照组、 FHF组、丹参组、促肝细胞生长素（ PHGF）组。 FHF、丹参组和PHGF组动物模型采用TAA皮下注射，剂量按每kg体重600mg，2次，每次间隔24h，复制FHF动物模型。丹参组和PHGF组动物除皮下注射TAA外，于实验前3天至实验结束分别于皮下注射丹参注射液1ml/100g和PHGF1ml/100g，正常对照组和FHF组大鼠同时皮下注射生理盐水1ml/100g。 FHF组、丹参组和PHGF组，于第2次注射TAA后24h，随机取大鼠各8只，腹主动脉取血测肝功能。迅速取肝组织，用10％甲醛液固定，制成石蜡切片，检测肝细胞有丝分裂指数（ MI）和增殖细胞核抗原（PCNA）。结果：丹参具有降低FHF大鼠ALT、 AST及TBil，显著提高肝细胞MI和PCNA的作用（P＜0.05， P＜0.01）。结论：丹参具有改善FHF大鼠肝功能和促进肝细胞增殖及再生的作用。