金属铜配合物的合成、晶体结构、抗癌活性及与牛血清白蛋白的相互作用

摘要

合成了一个含有席夫碱配体的四配位的铜配合物,通过元素分析、红外光谱对其进行表征并通过X射线单晶衍射仪测试它的结构,属于单斜晶系.此外,通过MTT法(MTT为3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐)测试了配合物对宫颈癌细胞、胃癌细胞、肝癌细胞和乳腺癌细胞的细胞毒性.结果表明铜配合物对4种肿瘤细胞的活性比顺铂高.通过荧光光谱法研究了配合物与牛血清白蛋白的相互作用,荧光光谱表明配合物与血清结合过程中发生了静态猝灭,并且计算了结合常数、结合位点数、结合距离和热力学常数△H,△S和△G.%A four-coordinated Cu(Ⅱ) complex with Schiff base ligand was synthesized and characterized by the elemental analysis and infrared spectroscopy,and the crystal structure was determined by X-ray diffraction.The crystal of complex belongs to monoclinic system.In vitro antitumor activities of Cu(Ⅱ) complex were evaluated by the 3-(4,5-dimethylthiazoly-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay against four human cancer cell lines (Hela,SGC-7901,HepG2,MCF-7).Cu(Ⅱ) complex exhibited stronger antitumor activity than carboplatin.The interaction of Cu(Ⅱ) complex with bovine serum albumin (BSA) was studied by spectroscopic method.The analysis of fluorescence data indicates the presence of static quenching mechanism in the binding process.Various binding parameters including binding constants,binding sites,binding distance and thermodynamic parameters AH,AS and AG have been evaluated.CCDC:1567198.