钌(Ⅱ)多吡啶配合物与DNA相互作用的等温滴定量热研究

摘要

The interaction of ruthenium(Ⅱ) polypyridyl complex with DNA has been studied by isothermal titrationcalorimetry (ITC). The results show that complex [Ru(phen)2PMIP]2+ {phen=1,10-phenanthroline, PMIP=2-(4-methylphenyl)imidazo[4,5-f]l,10-phenanthroline} interacts with calf thymus DNA (CT DNA) in terms of a modelfor a single set of identical sites through intercalation. The results are in agreement with our previous observationsfrom spectroscopic methods and viscosity measurements. In addition, the results further show that the drivingforce for DNA binding with the complex is mainly driven by the enthalpy changes, and the contribution from theentropy changes to this driving force is negligible.