目的 观察三物白散药物血清对人胃癌SGC-7901细胞凋亡及survivin mRNA表达的影响.方法 选择人胃癌细胞株SGC-7901,分为空白对照组、阳性对照组及三物白散低、中、高剂量组.三物白散药物血清干预SGC-7901细胞后,采用荧光染色法检测胃癌细胞的凋亡率,RT-PCR检测survivin mRNA的表达.结果 不同剂量组的三物白散药物血清干预SGC-7901细胞后,与空白对照组比较,各组细胞的凋亡率升高,且细胞凋亡率随药物血清浓度的增大、干预时间的延长逐渐升高;三物白散各剂量组survivin mRNA的表达均有不同程度的下降,与空白对照组比较,差异均有统计学意义(P＜0.05),且survivin mRNA表达随三物白散含药血清浓度的增大、干预时间的延长逐渐下降.结论 三物白散可诱导胃癌SGC-7901细胞凋亡,抑制survivin mRNA的表达.%Objective To investigate the effect of Sanwubaisan-containing serum on the apoptosis and survivin expression of human gastric cancer cell line SGC-7901. Methods Human gastric cancer cell line SGC-7901 cells were divided into normal group, low-dose Sanwubaisan group, medium-dose Sanwubaisan group, high-dose Sanwubaisan group, positive control group. The apoptosis was measured by fluorescence staining after treated by Sanwubaisan-containing serum, survivin mRNA expression was detected by RT-PCR. Results After the SGC-7901 cells treated by different concentrations of Sanwubaisan-containing serum, compared with the normal group, there was a increasing tendency on the apoptosis of SGC-7901 cells, depending on the concentration and time. There was a decreasing tendency in different degrees on the survivin mRNA expression after the SGC-7901 cells treated by different concentrations of Sanwubaisan-containing serum, with statistical significance (P<0.05) compared with the normal group, and decreased with the concentration and time. Conclusion Sanwubaisan-containing serum can induce apoptosis of SGC-7901 cells and inhibit survivin mRNA expression of gastric cancer SGC-7901 cells.
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