灭幽汤对幽门螺杆菌相关性胃炎脾胃湿热证小鼠热休克蛋白70和水通道蛋白4的影响

摘要

目的：观察灭幽汤对幽门螺杆菌(Hp)相关性胃炎脾胃湿热小鼠热休克蛋白70(HSP70)及水通道蛋白4(AQP4)的影响,探讨其作用机制。方法将小鼠随机分为灭幽汤高、低剂量组(灭高组、灭低组)和胃三联组、模型组、对照组,采用复合因素建立BALB/c小鼠Hp相关性胃炎脾胃湿热证模型,造模成功后连续给药14 d,灭高组、灭低组、胃三联组给药剂量分别为12.4、6.2 g/kg和0.2798 mg/kg；采用Western Blot检测HSP70、免疫组化法检测AQP4的表达情况。结果与对照组比较,模型组HSP70、AQP4蛋白表达显著增加(P＜0.05)；与模型组比较,灭高组、灭低组、胃三联组小鼠HSP70蛋白表达显著增加、AQP4蛋白表达显著降低(P＜0.01)。结论灭幽汤可能通过上调HSP70、下调AQP4蛋白表达而发挥治疗Hp相关性胃炎脾胃湿热证的作用。%Objective To study the expressions of HSP70 and AQP4 in the H.Pylori-associated gastritis mice of Pi-Wei hygropyrexia syndrome;To investigate the mechanism of Mieyou Soup. Methods The mice were randomly divided into high concentration of Mieyou Soup group, low concentration of Mieyou Soup group, gastric triad group, model group and control group. BALB/c mice of H.Pylori-associated gastritis mice of Pi-Wei hygropyrexia syndrome model was established by composite factor. After modeling, the mice were administered for of continuous 14 days. The drug dosage of high concentration of Mieyou Soup group, low concentrations of Mieyou Soup group, and gastric triad group were 12.4, 6.2 g/kg, and 0.279 8 mg/kg, respectively. Western Blot was used to detect the expression of HSP70 protein, and immunohistochemistry was employed to detect the expression of AQP4 protein. Results Compared with control group, the expression of HSP70 and AQP4 in model group significantly increased (P<0.05);compared with the model group, the HSP70 in high concentration of Mieyou Soup group, low concentrations of Mieyou Soup group, gastric triad group increased, the AQP4 in high concentration of Mieyou Soup group, low concentrations of Mieyou Soup group, gastric triad group decreased, with statistical significance (P<0.01). Conclusion Mieyou Soup may play a role in treatment of H.Pylori-associated gastritis mice of Pi-Wei hygropyrexia syndrome by raising the expression of HSP70, and reducing the expression of AQP4 protein expression.