糖痹康对糖尿病大鼠坐骨神经 p38丝裂素活化蛋白激酶及血浆肿瘤坏死因子-α表达的影响

摘要

目的：观察中药复方糖痹康对糖尿病大鼠坐骨神经 p38丝裂素活化蛋白激酶（p38 MAPK）及血浆肿瘤坏死因子-α（TNF-α）表达的影响，探讨其神经保护及镇痛机制。方法 SD 雄性大鼠60只，随机选取10只为正常组，其余大鼠采用高脂饲料和小剂量链脲佐菌素诱发2型糖尿病大鼠模型。成模后随机分为模型组、甲钴胺组和糖痹康低、中、高剂量组，每组10只。各给药组给予相应药物干预。16周后取大鼠一侧坐骨神经，放免法检测大鼠血浆 TNF-α含量；Western blot 检测大鼠坐骨神经 p38、p-p38 MAPK 蛋白表达。结果与正常组比较，模型组大鼠坐骨神经 p38、p-p38蛋白表达及血浆 TNF-α含量升高（P＜0.05，P＜0.01）；与模型组比较，各给药组大鼠血浆 TNF-α含量和坐骨神经 p-p38蛋白表达显著降低（P＜0.05，P＜0.01），各给药组 p38蛋白表达降低，但只有糖痹康高剂量组差异有统计学意义（P＜0.05）。结论糖痹康下调大鼠坐骨神经 p38、p-p38 MAPK 蛋白表达和血浆 TNF-α含量，可能是其糖尿病周围神经病变神经保护及镇痛作用靶点之一。%Objective To explore the effects of Chinese herbal compound Tangbikang on the expressions of p38 MAPK of sciatic nerve and plasma TNF-α in diabetic rats. Methods Ten of the sixty male SD rats were selected randomly as normal group, and the rest were fed with high-fat diet and low-dosage STZ was used to induce type Ⅱdiabetic rat models. Model rats were randomly divided into model group, mecobalamine group and Tangbikang low-, medium-, and high-dosage groups, 10 rats in each group. Each medication group was intervened with relevant medicine. Rat unilaterals sciatic nerves were taken after 16 weeks. The content of TNF-α in plasma was determined by radioimmunoassay. Western blot method was used to detect the expressions of p38 and p-p38 MAPK protein of sciatic nerve. Results Compared with normal group, the expressions of p38 and p-p38 protein and content of TNF-αin model group significantly increase (P<0.05, P<0.01). Compared with the model group, the expressions of p-p38 protein and the content of TNF-α significantly decreased after medicine intervention in different doses Tangbikang groups and mecobalamin group (P<0.05, P<0.01). The expression of p38 protein in Tangbikang high-dose group significantly decreased (P<0.05), with statistical significance (P<0.05). Conclusion Tangbikang can reduce the expression of p38 and p-p38 MAPK protein of the rat sciatic nerve, and reduce the content of TNF-α protein in rat plasma, which may be one of the effective targets of neuroprotection and abirritation of diabetic peripheral neuropathy.