1株利奈唑胺耐药粪肠球菌的基因突变检测

摘要

Objective To investigate the mechanism of linezolid resistance in a strain of Enterococcus faecalis. Methods We isolated a linezolid-resistant Enterococcus faecalis from the sputum in a patient with heamtological disease. The 23S rRNA V-re-gion and 4 copies of 23S rRNA gene fragments of the linezolid-resistant Enterococcus faecalis strain were amplified using poly-merase chain reaction (PCR), followed by DNA sequencing and sequence alignment analysis. Results One G→U mutation at the 2576 nucleotide (G2576U) was identified in the V-region of 23S rRNA of this drug-resistant strain. A G2576U mutation was also found in three out of the four copies of gene fragments of 23S rRNA. Conclusions G2576U point mutation may be the cause of resistance of this strain to linezolid.%目的 探讨1株对利奈唑胺耐药的粪肠球菌的耐药机制.方法 从广东医学院附属深圳南山医院1例血液病患者痰标本分离出1株对利奈唑胺耐药的粪肠球菌,采用聚合酶链反应(PCR)法,对该菌利奈唑胺耐药的粪肠球菌23S rRNA V区域和23S rRNA 4个拷贝基因片段进行扩增和测序比对分析.结果 耐药菌株23S rRNA V区域第2576位核苷酸发生G→U突变(G2576U),4个拷贝基因片段中,除拷贝1外,其他3个拷贝均发生G2576U突变.结论 G2576U点突变是该菌株的耐药机制.