替加环素、米诺环素对VanM型万古霉素耐药屎肠球菌体外抗菌活性

摘要

Objective To investigate thein vitro activity of tigecycline and minocycline against VanM-type vancomycin-resistant Enterococcusfaecium.Methods A total of 45 strains of VanM-type vancomycin-resistantE. faecium were obtained from hospitals in Shanghai between 2006 and 2014. Species and vancomycin resistance genotype were identiifed by PCR and sequencing analysis. Minimal inhibitory concentrations (MICs) of ten antimicrobial agents against these strains were determined.Results All the 45 isolates were VanM-type vancomycin-resistantE. faecium, and resistant to vancomycin (MIC: 128 to >256 mg/L). And 71.1%of the strains were resistant to teicoplanin. Almost all isolates showed resistance to levolfoxacin (100%) and ampicillin (97.8%). About 15.6%, 64.4% and 82.2% of the strains were resistant to minocycline, gentamicin and rifampicin, respectively.Conclusion Tigecycline and minocycline exhibit excellentin vitro activity against these VanM-type vancomycin-resistantE. faecium isolates.%目的：了解替加环素及米诺环素对VanM型万古霉素耐药屎肠球菌临床分离株的体外抗菌活性。方法收集2006—2014年上海地区临床分离VanM型万古霉素耐药屎肠球菌45株；采用PCR扩增及测序确认菌种和万古霉素耐药基因型；测定替加环素及米诺环素等10种抗菌药物对VanM型万古霉素耐药屎肠球菌的MIC。结果45株临床分离株均确认为VanM型万古霉素耐药屎肠球菌；所有菌株对万古霉素高度耐药（MIC 128～＞256 mg/L），对替考拉宁耐药率为71.1%。几乎所有菌株对左氧氟沙星（100%）和氨苄西林（97.8%）耐药，对米诺环素、庆大霉素和利福平的耐药率分别为15.6%、64.4%和82.2%，未检出替加环素和利奈唑胺耐药株。结论替加环素和米诺环素对VanM型万古霉素耐药屎肠球菌有良好的体外抗菌活性。