大蒜油对正己烷在小鼠体内代谢成2,5.己二酮的影响

摘要

目的 探讨大蒜油对正己烷(n-hexane)在小鼠体内代谢成2,5.己二酮(2,5.hexanedione,2,5-HD)的影响.方法 取健康成年昆明种小鼠,随机分为正己烷染毒组和大蒜油干预组,分别灌胃给予正己烷和大蒜油,染毒结束后取血,分离血清经乙酸乙酯萃取后气相色谱法测定血清中2,5-HD含量.结果 (1)一次性给予4000 mg/kg正己烷后,小鼠血清中2,5-HD含量随时间延长而增加,10 h后达峰值,20 h后几乎不能检出;(2)对照组小鼠血清中未检出2,5-HD;随着正己烷染毒量的增加,小鼠血清中2,5-HD含量明显增加,2000、4000和6000 mg/kg组染毒8 h后小鼠血清2,5-HD含量分别为8.04、16.68和22.38μg/ml,呈明显的剂量一效应关系;(3)不同周龄小鼠给予正己烷8 h后血清中2,5-HD含量有明显差异,5周龄组(22.83;μg/ml)分别大于4周(19.59μg/ml)和6周龄组(16.42μg/ml),差异有统计学意义(P<0.05);(4)不同性别小鼠给予同剂量正己烷后,雌性小鼠血清中2,5-HD含量(13.22 t,ga)高于雄鼠(10.34μg/ml),差异有统计学意义P<0.05;(5)正己烷染毒前后2 h分别给予80 mg/kg大蒜油,血清中2,5-HD含量与单纯染毒组相比均明显降低,差异有统计学意义(P<0.05),但染毒前给药组较染毒后给药组更低,但差异无统计学意义(P>0.05);染毒前2 h给予不同剂量大蒜油,10、20、40、80 mg/kg大蒜油+3 000 mg/kS正已烷组小鼠血清2,5-HD含量比染毒组分别降低16.2%、20.8%、22.8%和32.1%,差异有统计学意义(P<0.05,P<0.01),呈明显的剂量一效应关系.结论 在雌性小鼠血清中正己烷的代谢产物2,5-HD高于雄性;5周龄小鼠血清2,5-HD含量较4周和6周龄小鼠高;正己烷染毒前后给予大蒜油可明显减少2,5-HD的生成.%Objective To investigate the effects of garlic oil (GO) on n-hexane metabolized to 2,5-hexanedione(2,5-HD) in mice. Methods Adult healthy Kunming-mice were treated with n-hexane and GO. The serum was obtained and extracted with ethyl acetate, and the levels of the serum 2,5-HD were determined by gas chromatography. Results (1) The concentration of 2,5-HD in serum increased firstly after a single exposure to n-hexane (4 000 mg/kg). The peak value occurred at 10 hours after n-hexane treatment, but could hardly be detected at 20 h. (2) There was no 2,5-HD in serum of control mice. The content of 2,5-HD in serum increased along with the exposure dose of n-hexane. The serum 2,5-HD contents of the 2000, 4000 and 6000 mg/kg groups mice were 8.04, 16.68 and 22.38 |xg/ml at 8 h in pretreated mice, respectively, and showed significant dose-effect relationship.(3) When the different age mice were exposed to the same dose of n-hexane, the contents of 2,5-HD in serum were significantly different after 8 hours (P< 0.05). The serum 2,5-HD level of the 5 weeks old mice (22.83 M-gAiu1) was much higher than the 4 (19.59 fig/ml) and 6 (16.42 jig/ml) weeks old mice. (4) When the different gender mice were exposed to the same dose of n-hexane, the concentration of 2,5-HD in serum of female mice (13.22 |xg/ml) was higher than that of the female mice (10.34ug/ml, P<0.05). (5)GO significantly inhibited the increase of the serum 2,5-HD levels of both the pretreatment and post-treatment groups treated with 80 mg/kg n-hexane respectively, but the pretreatment with GO exhibited the more suppressive effects than the post-treatment (P>0.05). Compared with the n-hexane group, the concentrations of serum 2,5-HD in GO-pretreated groups mice decreased by 16.2%, 20.8%, 22.8%(P<0.05) and 32.1% (P<0.01), respectively, and showed significant dose-effect relationship. Conclusion The serum content of 2,5-HD, the metabolite of n-hexane, is different in different genders and age mice after exposed to the same dose of n-hexane. GO can effectively inhibit the production of n-hexane metabolized to 2,5-HD in mice serum.