Objective To investigate the influence of aluminum chloride(AlCl3) solution on the embryonic development of zebrafish and neurobehavior of juvenile fish.Methods The embryos of zebrafishat 6 hours after fertilization were exposed to AlCl3 solution at a concentration of 0,55.0,60.5,66.6,73.5,80.5,or 100.0 mg/L,and embryonic hatching rates at 48 and 72 hours after fertilization were calculated.The embryos of zebrafishat 6 hours after fertilization were exposed to AlCl3 solution at a concentration of 0,60.0,72.0,86.4,103.7,or 124.4 mg/L,and the embryonic mortality rates at 12,24,48,72,and 96 hours after fertilization were calculated.The embryos of zebrafish at 6 hours after fertilization were exposed to AlCl3 solution at a concentration of 0,50,100,200,400,or 800 μg/L,and the changes in the neurobehavior of juvenile fish were observed after hatching,including touch-escape reaction at 72 hours after fertilization and autonomic movement and panic escape reflex at 7 days after fertilization.Results Compared with the 0 mg/L group,the ≥66.6 mg/L group had a significant reduction in embryonic hatching rate at 48 and 72 hours after fertilization,and the ≥72.0 mg/L group had a significant increase in embryonic mortality rate at 96 hours after fertilization (P＜0.05).Compared with the 0 μg/L group,the ≥ 100 μg/L group had a significant reduction in the number of times of touch-escape reaction (P＜0.05).Compared with the 0 and 50 μg/L groups,the 100-800 μg/L groups had significant reductions in total movement distance and average speed (P＜0.05).Compared with the dark period before illumination,all groups had a significant increase in movement speed during the light period of the panic escape reflex test (i.e.,the third minute) (P＜0.05);within 2 minutes after the light was turned off,there was no significant change in movement speed in the 0-200 μg/L groups (P＞0.05);the 400 and 800 μg/L groups had a significant increase in movement speed (P＜0.05).Conclusion AlCl3 exposure may cause embryonic developmental disorder in zebrafish and abnormal neurobehavior in juvenile fish.%目的 探讨氯化铝(AlCl3)溶液对斑马鱼胚胎和幼鱼神经行为的影响.方法 将受精后6h的斑马鱼胚胎分别暴露于0、55.0、60.5、66.6、73.5、80.5和100.0 mg/L AlCl3溶液中,分别计算受精后48、72h的斑马鱼胚胎孵化率;将受精后6h的斑马鱼胚胎分别暴露于0、60.0、72.0、86.4、103.7和124.4 mg/L AlCl3溶液中,分别计算受精后12、24、48、72和96 h的斑马鱼胚胎死亡率;将受精后6h的斑马鱼胚胎分别暴露于0、50、100、200、400和800 μg/L AlCl3溶液中,待其孵化后观察幼鱼的神经行为变化,包括受精后72 h的幼鱼触动逃避反应、受精后7d的幼鱼自发运动以及惊恐逃避反射.结果 与0mg/L浓度组相比,≥66.6 mg/L浓度组斑马鱼受精后48、72 h胚胎孵化率明显下降,≥72.0 mg/L浓度组斑马鱼受精后96 h的胚胎死亡率明显增加,差异均有统计学意义(P＜0.05).与0 μg/L浓度组相比,≥100 μg/L浓度组斑马鱼幼鱼触动逃避次数明显减少,差异均有统计学意义(P＜0.05).与0和50 μg/L浓度组相比,≥100 μg/L浓度组斑马鱼总运动距离和平均速度均明显降低,差异均有统计学意义(P＜0.05).与光照前黑暗期相比,受精后7d各浓度组斑马鱼幼鱼惊恐逃避反射试验光照期(即第3分钟)运动速度均加快(P＜0.05);在关闭光源后2 min内,100和200 μg/L浓度组幼鱼运动速度与光照前黑暗期相比,差异无统计学意义(P＞0.05);400和800 μg/L浓度组幼鱼运动速度明显高于光照前水平,差异均有统计学意义(P＜0.05).结论 AlCl3暴露可能导致斑马鱼胚胎发育障碍以及幼鱼神经行为异常.
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