温阳活血通络方对SSc小鼠体内TGF-β1与MCP-1水平的影响

摘要

Objective:To observe the influence of Wenyang Huoxue Tongluo (WHT) medicine in mice with bleomycin-induced scleroderma(BIS) of TGF-β1 and MCP-1 levels.Methods:Bleomycin was injected into the subcutaneous tissue of mice to establish BIS models.The BIS mice were fed with WHT ,prednisone ,WHT plus prednisone or saline only as control for 28 days.The pathological changes in the skin and lung tissue of mice were evaluated with HE staining and Masson staining .The level of TGF-β1 and MCP-1 in serum was detected with ELISA ,as well as which in the skin tissue was detected with immunohistochemistry.Results: ①The location and amount of TGF-β1and MCP-1 in skin and lung tissue were different in BIS mice.Expression of TGF-β1 and MCP-1 in BIC mice skin tissue were significantly decreased in WHT or WHT +prednisone treated groups compared to no-treated ones ( P<0.05 , respectively),especially in WHT+prednisone one.②In serum of BIS mice,TGF-β1 and MCP-1 increased significantly than in normal control ( P<0.01 ).And after treated with WHT or WHT+prednisone ,the level of TGF-β1 and MCP-1 were significantly decreased ( P<0.05 ) compared with no-treated one ,especially in WHT+prednisone one.Conclusion: WHT can improve the pathological changes in skin and pulmonary tissue of BIS mice ,which might partially result from that WHT down-regulate TGF-β1 and MCP-1 in serum and skin tissue from BIS mice.%目的：观察温阳活血通络方对硬皮病小鼠体内TGF-β1、MCP-1水平的影响。方法：采用博来霉素皮下注射制造硬皮病小鼠模型，对模型组小鼠分别给予温阳活血通络方、醋酸泼尼松、二药联合、生理盐水灌胃28 d；HE染色与Masson染色观察皮肤与肺组织病理学改变，ELISA检测血清中TGF-β1与MCP-1的表达；免疫组化检测皮肤与肺组织中二者表达水平。结果：①TGF-β1与MCP-1在模型鼠皮肤组织与肺中的表达部位有一定差异。各治疗组TGF-β1与MCP-1较模型组均明显减弱（ P＜0.05），尤以两药联合治疗组变化明显。②模型鼠血清中TGF-β1与MCP-1表达较正常鼠均显著升高（ P＜0.01），治疗后，TGF-β1与MCP-1表达明显降低（ P＜0.05），尤以两药联合治疗组变化明显。结论：温阳活血通络方能够改善硬皮病小鼠皮肤与肺组织的病理学变化，且可能通过下调血清及皮肤组织中TGF-β1与MCP-1来发挥治疗作用。