人肝癌细胞基因组中HBV DNA的整合与c-fos、p53基因表达之间的关系

摘要

To investigate the actions HBV DNA, c-fos and p53 genes in theoccurrence of liver cacinoma and the relations among them. HBV DNA integration and expressions of c-fos and mutant p53 genes in liver cacinoma genomes were detected with PCR and immunohistochemical ABC method. The rate of HBV DNA integration is 67%, the positive rates of c-fos protein and mutant p53 protein are 67% and 58% respectively. There is signficant identity between HBV DNA integration and c-fos activation, p53 mutation (P＞0.05；P＞0.05). There is negative correlation between c-fos activation and p53 mutation, but there is no significant (r=-0.2816，P＞0.05). HBV DNA integration might cause c-fos activation and/or p53 mutation, and then lead to the occurrence of liver cacinama.%为了探讨HBVDNA、c-fos和p53在肝癌发生中的作用及其关系。利用PCR技术和免疫组化ABC法，检测了肝癌基因组中HBVDNA的整合、c-fos和突变p53的表达。HBVDNA整合率为67%，C-FOS蛋白阳性率为67%，突变P53蛋白阳性率为58%。HBVDNA整合与c-fos激活、p53突变有显著的一致性(P＞0.05；P＞0.05)，c-fos激活与p53突变之间呈负相关，但无显著意义(r=-0.2816，P＞0.05)。HBVDNA的整合可能引起c-fos的激活和/或p53的突变，从而导致肝癌的发生。