中央型和效应型记忆T细胞亚群在肝癌患者肿瘤浸润淋巴细胞中的分布

摘要

目的 研究中央型记忆T细胞(TCM)和效应型记忆T细胞(TEM)在肝癌肿瘤浸润淋巴细胞中的分布特点.方法 通过流式细胞仪检测肝脏组织浸润淋巴细胞CD3、CD4、CD8、CD45RO、CD62L、CCR7表面标志的表达情况.结果 乙肝肝癌组、乙肝癌旁组及对照组CD4+ TCM亚群占CD4+ CD45RO+ Tm的比例分别为(0.25±0.17)％、(0.29±0.22)％、(0.37±0.33)％,差异无显著统计学意义(P＞0.05).CD4+ TEM亚群占CD4+ CD45RO+Tm的比例分别为(89.7±7.4)％、(98.7±0.6)％、(95.5±3.7)％,差异有统计学意义(P＜0.05).其中乙肝肝癌组明显低于乙肝癌旁组及对照组.乙肝肝癌组、乙肝癌旁组及对照组CD8+ TCM亚群占CD8+ CD45RO+ Tm的比例分别为(0.41±0.31)％、(0.55±0.60)％、(0.26±0.25)％,其中乙肝癌旁组显著高于对照组,差异有统计学意义(P＜0.05).CD8+ TEM亚群占CD8+ CD45RO+ Tm的比例分别为(92.4±4.3)％、(98.1±0.7)％、(98.2±1.1)％,乙肝肝癌组显著低于乙肝癌旁组及对照组,差异有统计学意义(P＜0.05).结论 原发性肝癌患者肿瘤浸润CD45 RO+ Tm中,无论是CD4+还是CD8+,主要以TEM亚群为主,TCM亚群所占比例很小.CD4+ TEM亚群及CD8+ TEM亚群占CD45RO+ Tm的比例在肝癌组织中显著小于非肝癌组织,而CD4+ TCM亚群及CD8+RM亚群占CD45RO+ Tm的比例在肝癌组织及非肝癌组织中无差别.%Objective To illustrate the distribution of two memory T cells subsets CD45RO+CD62L+ CCR7 +T cells (central memory T cells,TCM) and CD45RO+ CD62L-CCR7-T cells (effector memory T cells,TEM) in tumor infiltrating lymphocytes (TILs) of hepatocellular carcinoma (HCC) patients.Methods Polychromatic flow cytometry (PFC) was used to test the cell surface molecules of infiltrating lymphocytes in liver,including CD3,CD4,CD8,CD45RO,CD62L and CCR7.Results The proportions of CD4 + TCM subsets in CD4 + CD45RO + Tm in HCC tissues,adjacent liver tissues and normal liver tissues were (0.25 ±0.17)％,(0.29 ±0.22)％ and (0.37 ±0.33)％,and there were no significant differences (P ＞ 0.05).The proportions of CD4 + TEM subsets in CD4 + CD45RO +Tm in HCC tissues,adjacent liver tissues and normal liver tissues were (89.7 ± 7.4) ％,(98.7 ± 0.6) ％ and (95.5 ± 3.7) ％,respectively.Paired comparison showed that the proportions of CD4 + TEM subsets in HCC tissues were significantly lower than those in adjacent liver tissues and normal liver tissues (P ＜ 0.05).The proportions of CD8 + TCM subsets in CD8 + CD45RO + Tm in HCC tissues,adjacent liver tissues and normal liver tissues were (0.41 ± 0.31) ％,(0.55 ± 0.60) ％ and (0.26 ± 0.25) ％.Paired comparison showed that the proportions of CD8 +TCM subsets in adjacent liver tissues were significantly higher than those in normal liver tissues (P ＜ 0.05).The proportions of CD8 + TEM subsets in CD8 + CD45RO + Tm from HCC tissues,adjacent liver tissues and normal liver tissues were (92.4 ±4.3)％,(98.1 ±0.7)％ and (98.2 ± 1.1)％.Paired comparison showed that the proportions of CD8 + TEM subsets in HCC tissues were significantly lower than those in adjacent liver tissues and in normal tissues (P ＜ 0.05).Conclusions Comparing to the TCM,TEM subsets including CD4 + and CD8 +,accounted for the majority of tumor infiltrating CD45RO + Tm in HCC patients.The proportions of CD4 + TEM and CD8 + TEM subsets in CD45RO+ Tm from tumor tissues were significantly lower than those in non-cancerous tissues.However,the proportions of CD4 + TCM and CD8 +TCM subsets in CD45RO + Tm from tumor tissues were the same as those from non-cancerous tissues.