核不均一核糖核蛋白A1的表达与肝癌肝移植患者肿瘤发生与转移的关系

摘要

Objective To study the expressions of HnRNPA1 in patients with recurrent or metastatic hepatocellular carcinoma (HCC) after liver transplantation.Methods The expressions of HnRNPA1 protein in pericarcinoma and normal liver tissues were detected using Western blot analysis in 16 patients with HCC.Immunohistochemical analysis was done in 141 patients with HCC.All these patients underwent liver transplantation.The relationship between the expressions of HnRNPA1 with recurrent and metastatic HCC were analyzed.Results The positive expression rate of HnRNPA1 protein in the HCC tissues (75.0％,12/16) was significantly higher than that in the pericarcinoma tissues (18.8％,3/16) (P ＜ 0.01).The expression of HnRNPA1 was positively correlated with tumor size,TNM type,vascular invasion and tumor encapsulation (P ＜ 0.01).Tumor recurrence in the HnRNPA1 high expression group was significantly higher than that in the HnRNPA1 low expression group (x2 =15.577,P ＜ 0.01).The survival rate was significantly lower in the high HnRNPA1 expression than that in the low expression group (x2 =6.309,P ＜0.05).Conclusion The expression of HnRNPA1 protein was a marker which predicted HCC recurrence or metastasis in patients after liver transplantation.%目的 探讨肝癌组织中核不均一核糖核蛋白A1(HnRNPA1)的表达与肝癌行肝移植治疗后肿瘤复发与转移的关系.方法 应用蛋白印迹法检测切除的肝癌组织、癌旁组织和肝正常组织中HnRNPA1的表达(n=16),用免疫组化方法分析其表达与移植后(n=141)肝癌复发及转移的关系和意义.结果 肝癌组织的HnRNPA1蛋白阳性率为75.0％ (12/16),癌旁组织为18.8％ (3/16),两者差异有统计学意义(P＜0.01).肝癌组织的HnRNPA1蛋白表达水平(0.680 ±0.149)明显高于癌旁组织(0.420±0.060)和正常肝组织(0.270 ±0.046,P＜0.01).HnRNPA1表达与肝癌直径、TNM分期、血管侵犯、肿瘤包膜等相关(P＜0.01),与肝癌其他临床病理学参数无关(P＞0.05).HnRNPA1高表达组患者肿瘤复发率明显高于HnRNPA1低表达组(x2=15.577,P＜0.01);生存率明显低于HnRNPA1低表达组(x2=6.309,P＜0.05).结论 HnRNPA1蛋白水平在肝癌组织中表达上调,且与肝癌肝移植术后肿瘤复发转移有关,影响肝癌肝移植患者预后.