大鼠背根神经节神经元转化生长因子-β1/Smad信号传导通路的实验研究

摘要

目的 探究大鼠背根神经节(dorsal root ganglia,DRG)神经元中是否存在转化生长因子β1/Smad信号通路.方法 人工培养大鼠背根神经节神经元,运用免疫荧光组织化学及实时荧光定量PCR技术研究DRG神经元中是否存在TGF-β1/Smad信号传导通路相关蛋白分子的表达以及TGF-β1信号对其基因转录的影响.结果 大鼠DRG神经元中存在TβR1、Smad2、Smad4等蛋白分子的表达,同时TGF-β1信号可以调控Smad分子基因的转录.结论 大鼠背根神经节神经元中存在受TGF-β1分子调控的Smad信号通路.%Objective To explore whether the TGF-β1/Smad signaling pathway exists in rat dorsal root ganglia neurons.Methods Rat dorsal root ganglia neurons were cultured ex vivo.Immunofluorescence histochemistry and real time fluorescence quantitative PCR were employed to detect the expression of Smad molecules in the rat DRG neurons and to test the influence of TGF-β1 on gene transcription of Smads.Results TβR1,Smad2 and Smad4 were expressed in DRG neurons of rats.The gene expression of these molecules in DRG neurons was regulated by TGF-β1.Conclusion The TGF β1/ Smad signaling pathway exists in rat dorsal root ganglia neurons.