目的 观察腺病毒介导反义微小RNA-21对裸鼠膀胱癌移植瘤的杀伤效应及毒性反应.方法 裸鼠膀胱接种人膀胱癌细胞株T24建立裸鼠膀胱癌移植瘤模型,经尿道膀胱内灌注重组腺病毒,自灌注始,每7 d测肿瘤体积,实验结束后处死裸鼠分离瘤体测量体积大小,噻唑蓝(MTT)比色法和原位末端标记(TUNEL)法分别检测肿瘤细胞增殖率和凋亡指数,常规病理学和生化检查评价肝肾毒性反应.结果 治疗组裸鼠肿瘤比未用药对照组生长明显缓慢,自实验对照的第1天算起至28 d实验结束处死裸鼠剥离出肿瘤,治疗组肿瘤体积显著小于未治疗组(P<0.05),两组间肿瘤细胞增殖率和凋亡率指数也存在显著差异(P<0.01);常规HE组织细胞染色病理检查以及血化验结果两组差异无统计学意义(P>0.05).结论 腺病毒介导的反义微小RNA-21在对裸鼠膀胱癌移植瘤生长发挥良好抑制作用的同时,对裸鼠肝肾无明显毒性反应,提示将微小RNA-21作为临床膀胱癌的靶向基因治疗可能具有一定价值.%Objective To observe killing effect and toxic reaction of adenovirus mediate anfisense microRNA-21 on transplanted tumor of bladder cancer of nude mice.Methods Transplanted tumor model of bladder cancer on nude mice were established by inoculating human bladder cancer cell line-T24 in its bladder.Then recombinant adenovirus was filled into bladder per urethra.Since filling, tumor volume was measured every 7 days.Then, the mice were sacrificed, and tumors were separated, and examined.Tumor cell growth rate and apoptotic index were measured by MTr and TUNEL Liver and renal toxicity were evaluated by routine pathology and biochemistry examination.Results The tumor growth of treated group was obviously slower than that of untreated control group.On 28 day, the tumor volume of treated group was statistically smaller than that of untreated group( P ＜0.01 ) ,and there were significant difference for the tumor cell growth rate and the apoptotic index between the two groups( P ＜0.01 ).There was no statistic significance for HE dying and biochemistry examination between the two groups(P＞0.05).Conclusions Adenovirus mediate antisense microRNA-21 has suppressing effect on nude mice transplanted tumor of bladder cancer.And at same time there is no obvious toxic effect on liver and kidney.This indicates that there might be some value to use microRNA-21 as a target gene treatment for bladder cancer.
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