老年皮肤萎缩的发病机制及对Th1/Th2细胞因子平衡的影响

摘要

Objective To investigate the pathogenesis of age-related atrophy of the skin and the effect on Th1/Th2 cytokines balance.Methods From January 2015 to October 2016,53 patients with senile skin atrophy were divided into observation group and treated with paeonol ointment.53 healthy older adults admitted to hospital at the same period were chosen as control group.Th1 cytokine interleukin-2,interferon-γ,Th2 cytokine interleukin-4 and interleukin-10 were detected by ELISA,and the self assessment symptom score scale was used to respectively detect symptoms condition before and after treatment and Th1/Th2 cytokine balance correlation.Results IL-2,interferon-γ of observation group were significantly higher than those of control group (P<0.05),and IL-4 and IL-10 of observation group were significantly lower than those of control group (P<0.05).After treatment 4 weeks,symptom of patients were improved,IL-2,interferon-γ level were significantly lower than those before treatment (P<0.05);IL-4 levels were significantly higher than those before treatment (P<0.05) in observation group.Senile skin atrophy symptoms score and IL-10 levels of Th1/Th2 cytokines had no significant difference (P>0.05) in observation group.There were correlation between senile skin atrophy symptom scores and Th1/Th2 cells factor IL-2,interferon-γ,IL-4 (P<0.05).Conclusions The pathogenesis of age-related skin atrophy is complex,Th1/Th2 cell imbalance shows that Th1 hyperfunction and Th2 cells decreasing.Paeonol ointment therapy could regulate Th1/Th2.%目的 观察老年性皮肤萎缩发病机制及对T淋巴细胞(Th)1/Th2细胞因子平衡影响.方法 老年性皮肤萎缩患者53例,设为观察组,行丹皮酚软膏治疗.选取同期入院健康体检老年53例,设为对照组.采用酶联免疫吸附法检测两组Th1细胞因子白细胞介素(IL)-2、干扰素-γ;Th2细胞因子IL-4及IL-10,采用自拟症状评分表评估患者治疗前后症状情况及Th1/Th2细胞因子平衡相关性.结果 观察组IL-2、干扰素-γ水平显著高于对照组(P<0.05);观察组IL-4及IL-10水平显著低于对照组(P<0.05);观察组经过丹皮酚软膏治疗4 w后患者症状得到改善,治疗后IL-2、干扰素-γ水平显著低于治疗前(P<0.05);观察组治疗后IL-4及IL-10水平显著高于治疗前(P<0.05);老年性皮肤萎缩患者症状评分与Th1/Th2细胞因子IL-10水平差异无统计学意义(P>0.05);老年性皮肤萎缩患者症状评分与Th1/Th2细胞因子IL-2、干扰素-γ、IL-4呈相关性(P<0.05).结论 老年性皮肤萎缩发病机制复杂,存在Th1/Th2细胞失衡,表现为Th1功能亢进及Th2细胞下降,利用丹皮酚软膏治疗能调节Th1/Th2细胞水平.