重组人松弛素对舒张功能不全模型兔心脏舒张功能的影响研究

摘要

Objective To study the effect of recombinant human relaxin (rh-RLX) on diastolic function in a rabbit diastolic heart failure (DHF) model.Methods Six out of the 30 New Zealand rabbits served as a sham operation group.A DHF model of 30 rabbits was established by abdominal aorta constriction.The animals underwent echocardiography at week 8 after operation and were divided into DHF group (n=6),benazepril (3 mg/kg · d) group (n=8),low rh-RLX dose (30 μg/(kg · d) group (n=8),high rh-RLX dose (98 μg/kg · d) group (n=8).After 1 week of drug treatment,their diastolic function was assayed by echocardiography and their myocardial fibrosis was detected with Masson staining at week 9 after operation.Results The E/A was significantly higher,the LVPW and IVS were significantly thinner,the LVEDP was significantly lower in high rh-RLX dose group than in DHF group (1.36±0.10 vs 0.83±0.06,P＜0.01;3.71±0.21 mm vs 4.87±0.08 mm,P＜0.01;3.10±0.08 mm vs 3.23±0.08 mm,P＜0.05;8.74±1.16 mm Hg vs 12.07±1.31 mm Hg,P＜0.01).The ratio of E/A was significantly higher,the LVPW was significantly thinner,the LVEDP was significantly lower in high rh-RLX dose group than in benazepril group (P＜0.05,P＜0.01).Conclusion High rh-RLX dose improves diastolic function in DHF rabbits by inhibiting their myocardial fibrosis.%目的 研究重组人松弛素(rh-RLX)对舒张性心力衰竭(DHF)模型兔心脏舒张功能的影响.方法 36只新西兰家兔随机选6只为假手术组,另30只腹主动脉缩窄术建立DHF模型兔,并随机分为DHF组6只,贝那普利组[3 mg/(kg·d)]8只,小剂量组[rh-RLX 30 μg/(kg·d)]8只,大剂量组[rh-RLX 98 μg/(kg·d)]8只,给药1周,术后第9周,行心脏超声心动图检查,Masson染色法检测心肌纤维化情况.结果 治疗后与DHF组比较,大剂量组二尖瓣舒张早期血流峰值/舒张晚期血流峰值(E/A,1.36±0.10 vs0.83±0.06,P＜0.01)、左心室后壁厚度(LVPW)[(3.71±0.21)mm vs (4.87±0.08)mm,P＜0.01]、室间隔厚度[(3.10±0.08)mm vs (3.23±0.08)mm,P＜0.05]、左心室舒张末压[LVEDP,(8.74±1.16)mm Hg vs (12.07±1.31)mm Hg,1 mm Hg=0.133 kPa,P＜0.01]均有显著差异;与贝那普利组比较,大剂量组E/A、LVPW及LVEDP有显著差异(P＜0.05,P＜0.01);与DHF组比较,贝那普利组、小、大剂量组心肌纤维化增生情况均有不同程度改善,大剂量组改善最显著.结论 大剂量rh-RLX可明显改善DHF兔心脏舒张功能,可能与rh-RLX的抗心肌纤维化有关.