背景:抑癌基因甲基化与食管癌相关,目前多种抑癌基因与肿瘤家族史相关性的报道少见.目的:研究多种抑癌基因在食管鳞状细胞癌(ESCC)中的甲基化状态及其临床意义.方法:选取2010年2～7月浙江省肿瘤医院76例ESCC患者.应用MSP技术检测肿瘤组织和相应癌旁正常组织中APC、RARβ2、CDH1、p16INK4a、RASSF1A等5个抑癌基因的甲基化状态,并分析抑癌基因甲基化状态与肿瘤家族史的关系及其对预后的影响.结果:ESCC组织APC、RARβ2、CDH1、p16INK4a、RASSF1A的甲基化率均显著高于相应癌旁正常组织(P＜0.05).ESCC组织中APC、RAR32、CDH1、RASSF1A甲基化与肿瘤家族史相关(P＜0.05);CDH1、RASSF1A甲基化患者的生存期明显低于非甲基化患者(P=0.015、P=0.016).结论:ESCC患者存在抑癌基因APC、RARβ2、CDH1、p16INK4a、RASSF1A高甲基化；且APC、RARβ2、CDH1、RASSF1A甲基化与肿瘤家族史显著相关,CDH1、RASSF1A甲基化患者的预后可能较差.%Background: There is a correlation between methylation of tumor suppressor genes and esophageal carcinoma, but studies on relationship between methylation of tumor suppressor genes and family history of carcinoma are few. Aims: To study the methylation of tumor suppressor genes and clinical significance in esophageal squamous cell carcinoma (ESCC). Methods: Seventy-six patients with ESCC from Feb. To July 2010 at Zhejiang Cancer Hospital were enrolled. MSP technique was used to detect methylation status of APC, RAR(32, CDH1, plG?4" and RASSF1A in tumor tissue and paracancerous normal tissue. Relationship of methylation status of tumor suppressor genes with family history of carcinoma was analyzed, and their influences on prognosis was estimated. Results: The methylation rates of APC, RAR|32, CDH1, pjfjiM?a and RASSF1A were significantly higher in tumor tissue than those in paracancerous normal tissue (P<0.05). Methylation of APC, RARp2, CDH1, RASSF1A genes in tumor tissue was significantly related with family history of carcinoma (P<0.05). The life span of patients with CDH1 or RASSF1A methylation was significantly lower than those without CDH1 or RASSF1A methylation (P=0.015, P=0.016). Conclusions: Hypermethylation of APC, RAR02, CDH1, p^?*1 and RASSF1A occurs in ESCC patients. Methylation of APC, RAR(J2, CDH1, RASSF1A genes is significantly correlated with family history of carcinoma. The prognosis of patients with methylation of CDH1 or RASSF1A is probably poor.
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