目的 研究肝癌细胞中XIAP与CDK4/CDK6/cyclin D1复合物之间的调控关系及在肝癌细胞增殖中的作用.方法 前期研究用Pathway Array进行肝癌组织异常表达蛋白筛选,后期XIAP特异性抑制剂embelin处理Huh7细胞进行增殖、周期分析.Western blot验证XIAP与CDK4/CDK6/cyclin D1复合物之间的调控关系.结果 Pathway Array提示XIAP与CDK4、CDK6、cyclin D1之间存在联合表达.embelin引起Huh7细胞G1期阻滞、S期分布减少,抑制细胞增殖.XIAP可以调节Huh7细胞G1期蛋白CDK4、CDK6、cyclin D1表达.结论 XIAP通过调节肝癌细胞G1期蛋白CDK4/CDK6/cyclin D1复合物的表达,促使细胞进入G1周期,从而促进其增殖.%Objective To investigate the relationship between XIAP and CDK4/CDK6/cyclin Dl complex in cell growth of hepatocellular carcinoma (HCC). Methods In preliminary study, Pathway Array was used to analyze protein in HCC tissues. After treatment of embelin in Huh7 cells, cell proliferation and cell cycle analysis were proceeded. Protein expression of XIAP, CDK4, CDK6 and cyclin Dl were detected by Western blot. Results Pathway Array showed co-expression of XIAP with CDK4, CDK6, cyclinDl in HCC tissues. Embelin could lead arrset of G, phase with concomitant decrease of S phases and inhibition of cell growth in Huh7 cells. XIAP could regulate expression of CDK4, CDK6 and cyclin Dl in Huh7 cells. Conclusion XIAP could promote cell proliferation through regulating proteins expression of CDK4, CDK6 and cyclin Dl in G, phase, leading cell entering to G, phase in HCC cells.
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