人偏肺病毒检测方法研究进展

摘要

人偏肺病毒(hMPV)是2001年在荷兰发现的一种副黏病毒,是婴幼儿、老年人和免疫功能受损患者中急性呼吸道感染的重要病原体.临床症状可表现为轻度上呼吸道感染到严重的下呼吸道疾病,包括细支气管炎和肺炎.实验室对hMPV检测主要采用直接免疫荧光法和RT-PCR法,根据不同的实验需求,需要采用不同的检测方法.本文对hMPV的检测方法进行了综述,以期为hMPV的进一步研究提供基础.%Human metapneumovirus (hMPV) is a newly described paramyxovirus that was found in the Netherlands in 2001. It is an important pathogen of acute respiratory diseases in infants, young children, elderly and immunocompromised patients. hMPV infection could cause mild upper respiratory tract infection or severe lower respiratory tract disease, including bronchiolitis and pneumonia. hMPV was usually detected using direct immunofluorescence and RT-PCR, but the detection method were different according to the respective experiment requirements. In this paper we review the detection method of hMPV to provide a basis for further study of hMPV.