慢性丙型肝炎合并非酒精性脂肪肝患者PBMC中TNF-α的研究

摘要

目的 通过研究慢性丙型肝炎合并非酒精性脂肪肝患者外周血单个核细胞(PBMC)中肿瘤坏死因子(TNF-α)的表达,初步探讨慢性丙型肝炎患者合并非酒精性脂肪肝的发病机制.方法 将20例慢性丙型肝炎合并非酒精性脂肪肝患者、20例单纯的慢性丙型肝炎患者及10例正常对照PBMC体外培养72 h后,用ELISA法检测培养上清中TNF-α的浓度.结果 (1)慢性丙型肝炎合并非酒精性脂肪肝患者组、单纯的慢性丙型肝炎患者组PBMC培养上清中TNF-α的水平较正常对照组明显升高(P<0.05).(2)慢性丙型肝炎合并非酒精性脂肪肝患者组PBMC培养上清中TNF-α的水平较单纯的慢性丙型肝炎患者组也明显升高(P<0.01).结论 TNF-α是导致慢性丙型肝炎患者合并非酒精性脂肪肝的一个重要因素.%Objective To study the roles of TNF-α secretion of PBMC in patients with chronic hepatitis C and Nonalcoholic fatty liver. Methods The level of TNF-α secretion of PBMC in patients with chronic hepatitis C and Nonalcoholic fatty liver was detected by ELISA after culturing for 72 hours in vitro, as well as patients with chronic hepatitis C and the normal control. Results (1) Compared with the normal control, the level of TNF-α in group with chronic hepatitis C and in group with chronic hepatitis C and Nonalcoholic fatty liver notably increased. (2) Compared with the group with chronic hepatitis C, the level of TNF-α in group with chronic hepatitis C and Nonalcoholic fatty liver also notably increased. Conclusion It suggested TNF-α takes important roles in the infection course of chronic hepatitis C with Nonalcoholic fatty liver.