IgA/EBV-VCA阳性人群和鼻咽癌患者的EB病毒潜伏膜蛋白2特异性细胞免疫及EBV DNA载量检测

摘要

Objective To investigate the correlation between EBV-LMP2-specific cell responses,EBV DNA copies and titers of IgA antibody to viral capsid antigen (IgA/VCA).Methods IgA/VCA antibody were tested with immune-enzyme method.Both of plasma and lymphocytes were respectively separated from IgA/VCA-positive individuals and patients with nasopharyngeal carcinoma (NPC) without treatment.EBV DNA copies and LMP2-specific cell responses were detected by interferon-gamma Elispots assay and fluorescent quantitative PCR method.Results IgA/VCA antibody in 80 of 1 233 individuals was positive in Cangwu county,and the positive rate is 6％.EBV-LMP2-specific cell responses and EBV DNA copies were detected in 72 of 80 IgA/VCA positive individuals and patients with NPC.EBV DNA copies in plasma were increased with rise of titers of IgA/VCA antibody,while EBV-LMP2-specific cell responses were declined.Conclusion EBV-LMP2-specific cell responses and EBV DNA copies are probably associated with titers of IgA/VCA antibody.%目的 探讨IgA/VCA抗体滴度与EBV LMP2特异性细胞免疫水平和EBV DNA拷贝数关系.方法 免疫酶法检测普查样本IgA/VCA抗体滴度;选取IgA/VCA抗体阳性人群和治疗前鼻咽癌患者,通过γ-干扰素Elispot和EBV DNA荧光定量试剂盒检测LMP2特异性细胞免疫水平和血浆中的EBV DNA载量.结果 普查广西苍梧县城自然人群共1 233人,其中80人IgA/VCA抗体阳性,阳性率为6.0％.对其中的72例IgA/VCA抗体阳性人群和20未治疗的鼻咽癌患者进行了LMP2特异性细胞免疫和EBV DNA检测,随着抗体滴度的升高,LMP2特异性细胞免疫反应水平降低,而血浆中EBV DNA拷贝数有随IgA/VCA抗体滴度升高而增加的趋势.结论 EBV-LMP2特异性细胞免疫水平和EBV DNA拷贝数可能与IgA/VCA抗体滴度相关.