目的 研究病毒共感染白纹伊蚊C6/36细胞后,白纹伊蚊浓核病毒-3(AalDV-3)对登革2型病毒(DENV-2)在细胞内的复制的影响.方法 C6/36细胞感染AalDV-3后5d,共感染DENV-2,设立单独感染AalDV-3与DENV-2为对照组.共感染后不同时间点,qPCR法检测病毒的拷贝数;共聚焦显微镜检测DENV-2感染率;共感染后37℃孵育24 h后,于1～2d以流式细胞术检测细胞凋亡率.结果 共感染后不同时间点,共感染组相对于DENV-2单独感染组,DENV-2基因拷贝数均明显下降(P＜0.05),其中第8d病毒滴度降低率达到最高的67％.共感染组DENV-2细胞阳性率均低于40％,明显低于DENV-2单独感染(＞70％,P＜0.05).流式细胞术结果显示,共感染组1～2d细胞凋亡率分别为23.43±1.02％和52.83±1.18％,明显低于DENV-2单独感染组凋亡率59.43±1.23％和96.21±1.87％ (P ＜0.05).结论 病毒共感染白纹伊蚊C6/36细胞后,AalDV-3可降低对DENV-2拷贝数,降低DENV-2细胞感染率,减少DENV-2诱导的细胞凋亡,AalDV-3具有防制蚊媒传染病的潜在价值.%Objective To study co-infection of a mosquito-specific Aedes albopictus densovirus-3 (AalDV-3) to interfere dengue virus type 2 (DENV-2) replication in mosquito cell.Methods Coinfected Ae.albopictus C6/36 cell with DENV-2 at 5 days post AalDV-3 infection,and DENV-2 and AalDV-3 single infected cell were used as control groups,respectively.The genome copy number of virus were quantified using real-time quantitative PCR (qPCR),percentage of DENV-2 positive C6/36 cells were determined by confocal microscope,apoptosis ratio of infected C6/36 cell were detected using flow cytometry at different time points post infection.Results The genome copy number of DENV-2 in coinfection group was significantly lower than that of single DENV-2 infection group at different time points post infection,and reached highest inhibitory ratio (67％) at 8 days post co-infection.DENV-2 antigen positive C6/36 cells of co-infection displayed significant lower percentage (＞ 40％) than that of single infection group (＞70％,P ＜0.05) at different time points post infection.The apoptosis ratio of C6/36 cell in co-infection group were 23.43 ± 1.02％ and 52.83 ± 1.18％ at 1 and 2 day post infection,that were lower than that of single infection group (59.43 ± 1.23％ and 96.21 ± 1.87％,P ＜ 0.05).Conclusions Co-infected Aedes albopictus C6/36 cell with AalDV-3 can down regulate the gene copy number of DENV-2 in cell,infected cell ratio and apoptosis ratio,and AalDV-3 has the potential as a tool for dengue control.
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