Objective To discuss the relationship between polymorphisms of lipoprotein-related phospholipase A2 (Lp-PLA2) and susceptibility of acute myocardial infarction (AMI). Methods AMI patients (AMI group, n=176) and healthy controls (control group, n=191) were chosen from the First Affiliated Hospital of Xi’an Jiaotong University College of Medicine from Jan. 2017 to Feb. 2018. After collecting serum samples, the single nucleotide polymorphism (SNP) of Lp-PLA2 gene at sits of rs1805017, rs16874954 and rs1051931 were detected by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The gene mutation of Lp-PLA2 and susceptibility of AMI were analyzed by using Logistic regression analysis. Results The genotypes of GG, GA and AA were detected at rs1805017 site, and genotypes of CC and AC were detected at rs16874954 site, and genotypes of AA, AC and CC at rs1051931 site. The distribution frequency of genotype AA at rs1805017 site and distribution frequency of genotype CC at rs16874954 site were higher in AMI group than those in control group (P<0.05). The level of triglyceride (TG) was higher, and level of high-density lipoprotein-cholesterol (HDL-C) was significantly lower in patients with genotypes GA+AA than those in patients with genotype GG at rs1805017 site (P<0.05). The level of TG was significantly higher in patients with genotype AC than that in patients with genotype CC at rs16874954 site (P<0.05). The other indexes of blood fat had no statistical difference among patients with different genotypes (P>0.05). The results of single-factor binary Logistic regression analysis showed that SNP at rs1805017 site (OR=0.788, 95%CI: 0.713~0.923, P<0.05) and rs16874954 site (OR=0.923, 95%CI: 0.859-0.983, P<0.05) were close correlated to AMI. The results of multi-factor Logistic regression analysis showed that polymorphism of rs1805017 site was independently correlated to AMI (OR=0.839, 95%CI: 0.804~0.975, P<0.05). Conclusion The polymorphisms at rs1805017 site and rs16874954 site of Lp-PLA2 are correlated to AMI occurrence, and SNP at rs1051931 site has no direct correlation with AMI.%目的 探讨脂蛋白相关磷脂酶A2(Lp-PLA2)基因多态性与急性心肌梗死(AMI)易感性之间的关系.方法 选取2017年1月~2018年2月于西安交通大学医学院第一附属医院住院的AMI患者176例(AMI组)以及健康体检志愿者191例(对照组),采集受试者血清标本,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测Lp-PLA2基因rs1805017、rs16874954、rs1051931位点单核苷酸多态性(SNP);采用Logistic回归分析Lp-PLA2基因突变与AMI易感性的相关性.结果 rs1805017位点检测到GG、GA、AA三种基因型;rs16874954位点检测到CC、AC两种基因型;rs1051931位点检测到AA、AC、CC三种基因型;AMI组rs1805017位点AA基因型以及rs16874954位点CC基因分布频率相对对照组更高(P＜0.05).rs1805017位点GA+AA基因型患者三酰甘油(TG)水平高于GG基因型患者,而高密度脂蛋白胆固醇(HDL-C)水平明显低于GG基因型患者(P＜0.05);rs16874954位点AC基因型患者TG水平明显高于CC基因型患者(P＜0.05);其余血脂水平在不同基因型患者之间并无统计学差异(P＞0.05).经单因素二元Logisitic回归分析,rs1805017(OR＝0.788,95%CI:0.713～0.923,P＜0.05)、rs16874954(OR＝0.923,95%CI:0.859～0.983,P＜0.05)位点SNP与AMI密切相关.多因素回归分析结果表明rs1805017位点多态性与AMI发病独立相关(OR＝0.839,95%CI:0.804～0.975,P＜0.05).结论本研究人群Lp-PLA2基因rs1805017、rs16874954多态性位点与急性心肌梗死的发生有关,而rs1051931位点SNP与AMI的发生可能无直接相关性.
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