重度冠状动脉钙化患者血浆白介素-37及骨保护素水平的变化及相关分析

摘要

Objective To investigate the changes of interleukin-37 (IL-37) and osteoprotegerin (OPG) in patients with severe coronary artery calcification (CAC), and analyze the correlation between them. Methods The patients with coronary heart disease (CHD) undergone coronary CT angiography (n=100, male 67, female 33, aged from 38 to 80 and average age=62.17±9.68) were chosen from Jan. 2015 to Dec. 2015. All patients were divided, according to Agatston integrals, into severe CAC group (Agatston integrals≥400) and control group (Agatston integrals<400, each n=50). The changes of biochemical indexes and level of high-sensitivity C-reactive protein (hs-CRP) were detected in all patients, and levels of plasma IL-37 and OPG were detected by using enzyme-linked immunosorbent assay (ELISA). Results In severe CAC group, more patients had hypertension and higher level of fasting plasma glucose (FPG) compared with control group (all P<0.05). The difference in age, sex, body mass index and blood fat had no statistical significance between 2 groups (all P>0.05). The levels of IL-37 [(89.67±23.84) pg/ml vs. (65.65±24.74) pg/mL], OPG [(6.95±1.76) pg/mL vs. (5.88±1.48) pg/mL] and hs-CRP [(1.92±1.44) mg/L vs. (1.28±0.88) mg/L] were significantly higher in severe CAC group than those in control group (all P<0.01). The results of Spearman’s correlation analysis showed that IL-37 level was positively correlated respectively to OPG level (r=0.320, P=0.001) and hs-CRP level (r=0.489, P<0.001), and OPG level was positively correlated hs-CRP level (r=0.191, P=0.057). Conclusion The increase of levels of IL-37 and OPG in patients with severe CAC indicates that IL-37 level is closely related to the process of CAC. The deepened study on IL-37 level and CAC may provide a new target for monitoring and treating CAC.%目的：探讨重度冠状动脉钙化（CAC）患者血浆白介素-37（IL-37）及骨保护素（OPG）水平的变化以及相关性分析。方法选择2015年1月~12月在首都医科大学附属北京安贞医院行冠状动脉CT血管造影检查的冠心病患者100例，其中男性67例，女性33例，年龄38～80（62.17±9.68）岁。依据Agatston积分进行分组，≥400分归为重度CAC组（50例），＜400分归为对照组（50例）。所有患者常规检测生化指标及高敏C反应蛋白（hs-CRP）水平，并采用酶联免疫法检测血浆IL-37和OPG水平。结果重度CAC组高血压比例高于对照组，空腹血糖更高，差异有统计学意义（P均＜0.05）。两组患者年龄、性别比、体质指数、血脂等其他指标比较，差异均无统计学意义（P均＞0.05）。重度CAC组血浆IL-37、OPG以及hs-CRP水平明显高于对照组，[（89.67±23.84）pg/ml vs.（65.65±24.74）pg/ml]，[（6.95±1.76）pg/ml vs.（5.88±1.48）pg/ml]，[（1.92±1.44）mg/L vs.（1.28±0.88）mg/L]，差异有统计学意义（P均＜0.01）。Spearman's 相关分析显示入选患者IL-37水平分别与OPG及hs-CRP水平呈正相关（r=0.320，P=0.001；r=0.489，P＜0.001），OPG水平与hs-CRP水平呈正相关（r=0.191，P=0.057）。结论重度冠状动脉钙化患者血浆IL-37及OPG水平升高，提示IL-37可能与冠状动脉钙化的发生发展密切相关，对IL-37和动脉钙化的深入研究有望为冠状动脉钙化监测和防治提供新靶点。