细胞内白细胞介素17A和白细胞介素6测定在不稳定型心绞痛中的临床意义

摘要

目的 探讨外周血单个核细胞胞内白介素-6(IL-6)、白介素-17A(IL-17A)在不稳定型心绞痛(UAP)中的作用及临床意义.方法 以湖北中医药大学第一临床学院心内科2016年5月～12月收入的UAP受检者85例、稳定型心绞痛(SAP)受检者50例、非冠心病受检者37例为研究对象,通过流式细胞仪,实时定量PCR,酶联免疫实验(ELISA)分别检测受检者发病就医后6 h,12 h外周血单个核细胞以及血清中IL-6、IL-17A水平并进行比较分析.结果 发病6 h内,与对照组相比,UAP组细胞内细胞因子IL-17A,IL-6水平都明显升高,差异均有统计学意义(P<0.05);发病12 h内,与对照组相比,UAP组细胞内细胞因子IL-17A,IL-6水平明显升高,差异均有统计学意义(P<0.05).发病6 h后,与对照组相比,UAP组细胞内与血清内细胞细胞因子IL-17A,IL-6水平均升高,差异均有统计学意义(P<0.05);与对照组相比,发病6 h内,SAP组细胞内与血清内细胞因子IL-17A,IL-6水平均升高,差异均有统计学意义(P<0.05).结论 炎症因子IL-6、IL-17A在UAP受检者中明显升高,特别是胞内细胞因子升高水平更加明显,可能更早反映了机体的炎症情况,但UAP发病机制复杂,对其诊断必须结合临床.%Objective To investigate the effects and clinical significance of intracellular cytokine-interleukin-6 (IL-6) and interleukin-17A (IL-17A) in peripheral blood mononuclear cells (PBMC) on unstable angina pectoris (UAP).Methods UAP patients (UAP group,n=85), patients with stable angina pectoris (SAP group,n=50) and controls without coronary heart disease (control group,n=37) were chosen from the First School of Clinical Medicine of Hubei University of Chinese Medicine from May 2016 to Dec. 2016. The levels of PBMC, IL-6 and IL-17A were detected and compared by using flow cytometer, RT-PCR and ELISA after patients hospitalized for 6 h and 12 h.Results Within 6 h after disease attack, levels of intracellular IL-17A and IL-6 increased significantly in UAP group compared with control group (P<0.05). Within 12 h after disease attack, levels of intracellular IL-17A and IL-6 increased significantly in UAP group compared with control group (P<0.05). After disease attack for 6 h, levels of intracellular and serum IL-17A and IL-6 increased in UAP group compared with control group (P<0.05). Within 6 h after disease attack, levels of intracellular and serum IL-17A and IL-6 increased in SAP group compared with control group (P<0.05).Conclusion The levels of IL-6 and IL-17A increase in UAP patients, especially their intracellular levels, which reflects possibly inflammation in the body. But the pathogenesis of UAP is complex and diagnosis on it should be combined with clinical practice.