短期高脂饮食引起大鼠下丘脑PGC1α表达下调

摘要

目的 研究短期高脂饮食对大鼠下丘脑过氧化物酶增殖体激活受体辅激动子-1α(PG C1α)表达的影响。方法18只SD雄性大鼠随机分为对照组（NC组）和高脂组（HF组）。适应性喂养2周后，分别给予基础饲料和高脂饲料喂养3d，每天记录体重和能量摄入量，测定干预前后尾静脉随机血糖。分离下丘脑，用定量RT-PCR和Western blot检测PGC1α，UCP2及CPT1a mRNA和蛋白表达水平。结果干预喂养3 d后，HF组大鼠每日摄入的能量明显高于NC组（75.70±5.02 vs60.40 +4.53 kcal/d,P＜0.05），差异有统计学意义，体重、每日摄食量及血糖水平差异无统计学意义。PGC1α mRNA明显下调（P＜0.05），蛋白水平无明显变化。结论短期高脂饮食可引起大鼠下丘脑PGC1α mRNA表达下调。%Objective To explore the effects of short-term high-fat diet on expression of peroxisome proliferator-activated receptor-γ-coactivator 1α (PGC1α) in hypothalamus of rats. Methods 18 male SD rats were randomly divided into 2 groups: the normal control group (NC) and the high-fat group (HF). After two-week adaptive feeding, the 2 groups were given control diet and high-fat diet respectively for 3 days. Body weight and energy intake were recorded every day. Random blood glucose of caudal vein was measured before and after intervention. The mRNA and protein levels of PGC1α, UCP2 and CPT1a in hypothalamus were detected by quantitative RT-PCR and Western Blot. Results After the three-day treatment, the energy intake in HF group was significantly increased compared with that in NC group (75.7 ±5. 02 vs 60.4 ±4.53 kcal/d, P ＜0.05). However,the body weight, food intake and blood glucose level were not significantly changed. The mRNA level of PGC1α was significantly decreased (P ＜0.05 ), while no significant difference was observed in protein levels. Conclusion Short-term high-fat diet downregu]ates the expression of PGC1α mRNA in hypothalamus of rats.