Objective To investigate the relationship between plasma glucose transporter (Glut) expression and ERK in diabetic patients with obesity. Methods Subjects in this study included type 2 diabetes (T2DM ) with overweight or obesity(T2DM+ OB/OW group ,n=20) ,T2DM with normal weight (T2DM group ,n=22) ,normal glucose with overweight or obesity(OB/OW group ,n=20) ,normal glucose with normal weight (NC group ,n=21). Automatic biochemical analyzer was used to detect the glucose concentrations ;colorimetry was used to detect blood lipid;ELISA was used to detect the level of insulin;Western blot was used to evaluate the expression of Glut1 Glut2 and Glut4 ;RT‐PCR was used to evaluate the mRNA expression of Glut2 ,Glut4 and ERK. Results Fasting plasma glucose (FPG )and two‐hour postprandial plasma glucose (2 hPG)in group T2DM+ OB/OW[(8.9 ± 3.7) ,(20.1 ± 3.5)mmol/L]and T2DM[(9.3 ± 2.8) ,(18.48 ± 4.59)mmol/L]were higher than in group OB/OW[(4.10 ± 0.90) ,(6.60 ± 0.75)mmol/L ]and NC[(3.9 ± 0.7) ,(6.7 ± 0.8)mmol/L](P<0.01).Hemoglobin A1c(HbA1c) in group T2DM+ OB/OW [9.90 ± 3.19)% ] and T2DM [(8.68 ± 2.75)% ] were higher than in group OB/OW [5.05 ± 0.37)% ] and NC[(5.47 ± 0.59)% ](P<0.01).FIns in group OB/OW [(7.98 ± 2.01)μIU/ml] were higher than in T2DM group[(5.91 ± 3.02)μIU/ml]and NC[(5.49 ± 1.36)μIU/ml](P< 0.05).Total cholesterol and triglyceride in T2DM + OB/OW group[(5.06 ± 0.97) ,(3.52 ± 2.68)mmol/L] , T2DM[(5.13 ± 1.27) ,(3.79 ± 3.56)mmol/L]and OB/OW[(4.67 ± 0.83) ,(1.87 ± 0.95)mmol/L]were higher than in group NC[(3.51 ± 0.89) ,(1.41 ± 1.21)mmol/L](P<0.01). Glut1 Glut2 ,Glut4 expression in T2DM+OB/OW group ,T2DM were lower than those in group OB/OW ,NC.Glut2 expression in T2DM+ OB/OW group was significantly lower than in T2DM group.Glut2 and Glut4 mRNA expression in group T2DM+ OB/OW ,T2DM were significantly lower than in group OB/OW ,NC.ERK mRNA expression in group T2DM + OB/OW ,T2DM were significantly higher than those in group OB/OW and NC.Conclusion Glut1 ,Glut2 and Glut4 expression are lower in diabetic patients with obesity ,and there is a negative correlation between the expression of ERK and Glut2 and Glut4.%目的：探讨肥胖T2DM患者血浆中葡萄糖转运蛋白（Glut）的表达及其与细胞外信号调节激酶（ERK）的关系。方法将研究对象分为T2DM合并超重／肥胖（T2DM＋ OB／OW）组、单纯T2DM （T2DM ）组、糖调节正常合并超重／肥胖（OB／OW）组及正常对照（NC）组。采用全自动生化分析仪测定血糖浓度；ELISA检测血胰岛素含量；比色法检测血脂四项及 HbA1 c水平；Western blot检测Glut1、Glut2和Glut4蛋白的表达；RT‐PCR检测 Glut1、Glut2、Glut4和 ERK mRNA 的表达。结果 T2DM ＋ OB／OW组及 T2DM 组 FPG、2 hPG、HbA1c水平[FPG ：（8.90±3.70），（9.30±2.80）mmol／L vs（3.90±0.70），（4.10±0.90）mmol／L ；2 hPG ：（20.10±3.50），（18.48±4.59）mmol／L vs（6.70±0.80），（6.60±0.75）mmol／L ；HbA1c：（9.9±3.19）％，（8.68±2.75）％ vs（5.05±0.37）％，（5.47±0.59）％]均高于OB／OW组及NC组（P＜0.01）；T2DM ＋ OB／OW、T2DM 及OB＋ OW组 TC和 TG水平[TC ：（5.06±0.97），（5.13±1.27），（4.67±0.83） vs（3.51±0.89）mmol／L ；TG ：（3.52±2.68），（3.79±3.56），（1.87±0.95） vs（1.41±1.21）mmol／L]均高于NC组（P＜0.01）；OB／OW组FIns水平[（7.98±2.01）vs（5.91±3.02），（5.49±1.36）mmol／L]高于T2DM、NC组（P＜0.05）。 T2DM＋ OB／OW、T2DM 组Glut1，Glut2， Glut4表达均低于OB＋ OW、NC组（P＜0.01）；T2DM ＋ OB／OW组Glut2表达低于 T2DM 组；T2DM ＋OB／OW、T2DM组Glut2和Glut4的mRNA表达低于 OB／OW、NC组；T2DM ＋ OB／OW、T2DM 组 ERK的mRNA表达高于OB／OW、NC组。结论肥胖T2DM患者体内Glut1、Glut2和Glut4的表达均降低，其中，Glut2和Glut4的表达与ERK的表达呈负相关。
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