黄芩苷对UVB诱导人皮肤成纤维细胞光产物生成的干预作用

摘要

Objective To observe the inhibitory effect of Baicalin on formation of the photoproduct in human skin fibroblasts induced by ultraviolet B (UVB) light. Methods Human skin fibroblasts were incubated with various concentrations of Baicalin (0-250μg/mL) for 24 hours. Immunohistochemistry and immune dot blot test (IDBT) was used to detect cyclobutane pyrimidine dimmers (CPDs) in DNA of human skin fibroblasts 30 minutes after 30mJ/cm2 UVB radiation and Baicalin intervention. The UV absorbance ability of Baicalin was detected by UV spectrophotometer. Results Baicalin could reduced CPDs formation 30 minutes after UVB radiation. Baicalin had a strong ability to absorb ultraviolet energy within UVA, UVB and UVC band. Conclusion Baicalin can inhibit the the formation of the photoproducts induced by UVB light in human skin fibroblasts. Baicalin is an effective UV protectant.%目的 观察中波紫外线(UVB)诱导人皮肤成纤维细胞光产物的形成情况,以及黄芩苷的干预作用.方法将各种不同浓度的黄芩苷(0～250μg/mL)与人皮肤成纤维细胞共同孵育24h后,采用免疫组织化学法及免疫斑点印迹技术检测30mJ/cm2UVB辐射及黄芩苷干预下,辐射后30min人皮肤成纤维细胞DNA内环丁烷嘧啶二聚体( CPDs)的产生情况.并采用紫外分光光度计法检测黄芩苷在紫外波段的吸光度.结果黄芩苷能在UVB辐射后30min内减轻光产物的形成,其在UVA、UVB和UVC波段内有较强的吸收紫外线能量的能力.结论黄芩苷对UVB诱导人皮肤成纤维细胞光产物的形成有一定抑制作用.黄芩苷是一种有效的紫外线防护剂.