原发性肝癌患者肿瘤浸润T淋巴细胞亚群分析

摘要

目的:了解原发性肝癌患者肿瘤浸润T淋巴细胞中CD4+及CD8+的表达特点.方法:收集30例原发性肝癌患者(均有乙型肝炎病毒感染背景)的癌组织(乙肝肝癌组)和癌旁组织(乙肝癌旁组)以及30例因良性病变而行肝切除的患者的新鲜肝组织(对照组),分离组织浸润淋巴细胞,用抗CD3、CD4和CD8单克隆抗体同时荧光染色,用流式细胞仪检测各表面标志的表达情况.结果:1)乙肝肝癌组、乙肝癌旁组及对照组CD3+CD4+T细胞占浸润淋巴细胞的比例分别为(22.31±3.68)％、(10.69±2.47)％及(4.21±4.26)％.乙肝肝癌组显著高于乙肝癌旁组及对照组,差异有统计学意义(P=0.000,P=0.001),乙肝癌旁组高于对照组,差异亦有统计学意义(P=0.019).2)乙肝肝癌组、乙肝癌旁组及对照组CD3+CD8+T细胞占浸润淋巴细胞的比例分别为(26.10±5.82)％、(21.82±2.70)％及(41.31±14.01)％,乙肝肝癌组及乙肝癌旁组显著低于对照组,差异有统计学意义(P=0.014,P=0.004),而乙肝肝癌组与乙肝癌旁组之间差异无统计学意义(P=0.651).3)乙肝肝癌组组织浸润淋巴细胞中CD3+CD4+T细胞与CD3+CD8+T细胞的比值为0.91±0.30,显著高于乙肝癌旁组(0.47±0.11,P=0.003)及对照组(0.11,0.13,P=0.000),CD3+CD4+与CD3+CD8+T细胞的比值出现失衡.结论:原发性肝癌患者肿瘤浸润淋巴细胞中T细胞亚群失调,表现为CD3+CD4+T细胞所占比例升高,CD3+CD8+T细胞所占的比例下降,CD3+CD4+/CD3+CD8+明显升高.%Objective:To investigate the changes of tumor infiltrating T lymphocyte subsets in HCC patients.Methods:Obtained fresh liver tissues from thirty patients with primary liver cancer and thirty patients who underwent hepatectomy because of benign lesions,then prepared infiltrating lymphocytes from these tissues.Used the method of flow cytometry to detect the cell surface molecules of T lymphocytes:CD3,CD4 and CD8.Results:1)The proportions of CD3+CD4+ T lymphocytes in infiltrating lymphocytes in HCC tissues were (22.31 ± 3.68)％,which were significantly higher than those in adjacent liver tissues (10.69 ± 2.47) ％ and in normal liver tissues (4.21 ± 4.26) ％ (P=0.000,P=0.001).The proportions of CD3+CD4+ T lymphocytes in adjacent liver tissues were significantly higher than those in normal liver tissues too (P=0.019).2)The proportions of CD3+CD8+T lymphocytes in infiltrating lymphocytes in HCC tissues and in adjacent liver tissues were (26.10 ± 5.82)％ and(21.82 ± 2.7)％,which were significantly lower than those in normal liver tissues (41.31 ± 14.01)％ (P=0.014,P=0.004).The proportions of CD3+CD8+ T lymphocytes were similar between those in HCC tissues and in adjacent liver tissues (P=0.651).3)The ratio of CD3+CD4+ T lymphocytes and CD3+CD8+T lymphocytes in infiltrating lymphocytes in HCC tissues(0.91 ± 0.30) were significantly higher than those in adjacent liver tissues(0.47 ± 0.11,P=0.003)and in normal liver tissues(0.11 ± 0.13,P=0.000).Conclusion:The proportions of CD3+CD4+ T lymphocytes in tumor infiltrating lympho cytes of HCC tissues increased evidently,while the proportions of CD3+CD8+T lymphocytes decreased markedly.The ratios of CD3+CD4+/CD3+CD8+ T lymphocytes were evidently raised.