腹腔复苏对感染性休克大鼠内脏器官保护效应的实验研究

摘要

Objective To investigate the protective effects of direct peritoneal resuscitation on internal organs in rats after septic shock. Methods The model of septic shock in rats was induced by intravenous LPS( 15 mg/kg). In 46 rals, 36 successfully replicaled model rals were randomly divided into 3 groups; intravenous resuscitation ( group Ⅰ ) , intravenous resuscitation and intraperitoneal salin (group Ⅱ ) and intravenous resuscitation and intraperitoneal 2. 5% clinical periloneal dialysis solution (group Ⅲ ). One hour after Intravenous injection of LPS, all rats were rapidly administrated with compound sodium lactale solution (25 mL/kg) for 30 minutes to maintain blood flow in vivo with high power levels. Then the animals in group Ⅰ withoul further treatment, animals in Ⅱ group were intraperiloneally injected wilh saline 100 mL/kg, animals in Ⅲ grooup were intraperiloneally injected wilh equal dosage of 2.5% clinical hypertonic low calcium dialysale. Two hours after resuscilalion, the level of arterial blood lactate concentration in serum was measured. Twenty hours after resuscilalion, the level of MDA, SOD and IL - 6 in visceral tissue and tissue dry/weight ratio were delected; finally, the histopalhology of livers and inteslines were observed. Results Compared with the control group, the level of arterial blood laclate concentration in serum and the level of MDA in visceral tissues were decreased, and the activity of SOD in visceral tissue was increased, and the level of IL - 6 in visceral tissue were decreased, and the degree of the edema and morphological damage of visceral lissue was alleviated. Conclusion Periloneal hypertonic fluid resuscilalion may help reduce the visceral injury by ischemia and hypoxia in rats after septic shock, played a proleclive role on inlernal organs in rats after septic shock.%目的 研究腹腔复苏对感染性休克大鼠内脏器官的影响.方法 46只健康SD雄性大鼠静脉推注内毒素复制感染性休克模型,将模型复制成功后的36只大鼠随机分为单纯静脉复苏(Ⅰ)组、腹腔生理盐水对照(Ⅱ)组和腹腔高渗液体复苏(Ⅲ)组.静脉注入LPS后1 h为复苏开始点,先用复方乳酸钠溶液(25 mL/kg)快速静脉复苏30 min,以维持体内高动力血流水平.而后Ⅰ组动物无处理,Ⅱ组动物腹腔注入生理盐水100 mL/kg,Ⅲ组动物注入等量2.5%临床高渗低钙腹膜透析液.观察各组动物复苏后2 h动脉血乳酸浓度;20 h取肝、小肠、肺等内脏组织检测干湿质量比、细胞因子IL-6含量、MDA含量、SOD活性,并作肝、肠组织病理切片HE染色.结果 与对照组比较,腹腔复苏能更大程度降低大鼠动脉血乳酸浓度,减少大鼠内脏组织MDA含量,提高内脏组织SOD活性,抑制大鼠内脏组织炎性因子IL-6的释放,改善内脏组织水肿程度和组织形态学损伤.结论 腹腔高渗液体复苏能帮助改善感染性休克大鼠内脏缺血缺氧导致的损害,起到保护感染性休克大鼠重要内脏器官的作用.