消癌平注射液下调MMP-9基因表达抑制卵巢癌细胞Caov-3迁移机制的研究

摘要

目的:研究中药消癌平注射液下调MMP-9基因表达,抑制卵巢癌细胞Caov-3迁移的机制.方法:利用消癌平注射液干预体外培养的卵巢癌Caov-3细胞,分别应用:细胞划痕实验观察细胞的迁移水平;肿瘤细胞侵袭实验(Transwell小室法)观察细胞侵袭能力;RT-PCR实验检测卵巢癌细胞中金属蛋白酶MMP-9基因表达水平.以金属蛋白酶MMPs特异性抑制剂-Doxycyclin作为阳性对照,同时应用PBS作空白对照.结果:消癌平可有效抑制Caov-3细胞迁移,与空白对照组相比差异有统计学意义(P<0.05),但与Doxycyclin组差异无统计学意义(P>0.05);消癌平还可部分抑制血清诱导的Caov-3细胞穿过Transwell小室,其抑制效果与Doxycyclin相似;此外,消癌平还能够下调MMP-9基因表达水平.结论:消癌平下调MMP-9基因表达,抑制卵巢癌Caov-3细胞迁移.%Objective: To study the inhibitory mechanism of an injection of the Chinese medicine Xiaoaiping ( XAP ) on the migration of human ovarian cancer Caov-3 cells via reduced matrix metalloproteinase-9 ( MMP-9 ) gene expression Methods: The ovarian cancer Caov-3 cells were treated with XAP in vitro. The inhibitor doxycyclin was also applied to the MMPs as the positive control, whereas phosphate-buffered saline served as the blank control. Wound healing and cell invasion assays using Transwell chambers were used to investigate the antimetastatic activities of XAP. The MMP-9 expression was detected via real-time polymerase chain reaction and Western blot analysis. Results: XAP effectively inhibited Caov-3 cell migration and invasion and decreased the MMP-9 gene and protein expression levels (P< 0.05 ). Moreover, the inhibitory effect of XAP was similar to that of doxycyclin (P> 0.05 ). Conclusion: XAP inhibits Caov-3 cell migration by decreasing the MMP-9 expression.