目的：探讨miR-21与肾癌转移的相关性，及miR-21对肾癌Caki-1细胞侵袭能力的影响。方法：实时PCR检测原发未转移肾癌和原发伴转移肾癌组织中miR-21的表达。将miR-21的前体pre-miR-21和抑制物anti-miR-21分别转染肾癌Caki-1细胞，实时PCR验证转染效果，然后检测转染后细胞的侵袭能力。结果：与原发未转移肾癌相比，原发伴转移肾癌组织中miR-21的表达显著上调；pre-miR-21和anti-miR-21转染后能够显著升高和降低Caki-1细胞miR-21的表达量；pre-miR-21组穿透滤膜的细胞数明显增加，而anti-miR-21组穿透滤膜的细胞数明显减少。结论：miR-21与肾癌的侵袭转移相关，miR-21能够促进肾癌细胞侵袭，在肾癌中具有促进转移的作用。% Objective: The present study aims to investigate the relationship between miR-21 and renal cancer metastasis as well as the influence of miR-21 on the invasiveness ability of renal cancer Caki-1 cells. Methods: Real-time PCR was performed to detect miR-21 in primary renal cancer without and with metastasis. Pre-miR-21 and anti-miR-21 were transfected to Caki-1 cells, respectively, and real-time PCR was used to detect transfection effects. Finally, invasiveness changes in Caki-1 cells were detected after transfection. Results: miR-21 expression in primary renal cancer with metastasis was much higher than that in primary renal cancer without metasta-sis. After transfection, pre-miR-21 and anti-miR-21 significantly increased and decreased the miR-21 expression in Caki-1 cells, respec-tively. The transmembrane cells of the pre-miR-21 group increased significantly, whereas those of the anti-miR-21 group decreased sig-nificantly. Conclusion: miR-21 and renal cancer metastasis are related. miR-21 advances renal cancer cell invasion and metastasis.
展开▼
