磁共振波谱成像观察大鼠大脑中动脉闭塞的针刺治疗效果

摘要

Objective:To observe the treatment effect of acupuncture on middle cerebral artery occlusion (MCAO) in rats through magnetic resonance spectroscopy (MRS) based on magnetic resonance imaging (MRI).Methods:A total of 60 male Sprague-Dawley rats were randomly divided into acupuncture group (27 cases),model group (27 cases) and sham operation group (6 cases).The acupuncture group and model group were further randomly divided into three subgroups (9 cases in each subgroup):24 h subgroup,3 d subgroup and 7 d subgroup.Rat MCAO model was constructed by modified inserting thread method.MRI and 1 H-MRS were examined at the corresponding time points,and the images were further processed and quantitatively analyzed.Results:After MCAO model construction,the lesion areas with high T2WI signal intensity in 7 d subgroup had a narrowing tendency compared with 24 h subgroup and 3 d subgroup.No obvious abnormal signals were observed in the sham operation group.1 H-MRS assay results showed that there were significant increases of N-acetyl aspartate (NAA) in 3 d subgroup and 7 d subgroup of acupuncture group compared with those of model group (31.43 ± 2.88 vs.25.90 ± 3.48,60.84 ± 2.76 vs.54.31 ± 1.40,both P＜0.05),but lactate (Lac) was decreased (58.82 ± 3.78 vs.65.15 ± 3.58,50.22 ± 4.33 vs.55.46 ± 4.06,both P＜0.05).However,no significant differences were found in choline (Cho) and creatine (Cr) (P＞0.05).Conclusions:1 H-MRS can objectively assess the intervention effect of acupuncture treatment on the lesions of rats with MCAO,and it is a reliable imaging method for researches on acupuncture treatment in cerebral ischemic stroke.%目的:采用基于磁共振成像(magnetic resonance imaging,MRI)的磁共振波谱成像(magnetic resonance spectroscopy,MRS)观察针刺对大鼠大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)的治疗效果.方法:将60只雄性SpragueDawley(SD)大鼠随机分为针刺组(n=27)、模型组(n=27)、假手术组(n=6),针刺组和模型组再按MRI检查时段分为3个亚组(每个亚组9只):24 h亚组、3d亚组和7d亚组.采用改良线栓法构建MCAO模型,在造模后行MRI、氢质子磁共振波谱成像(1H-MRS)检测,并对图像进行处理和定量分析.结果:MCAO模型7d亚组的大鼠栓塞区T2WI高信号范围较24 h亚组和3d亚组有缩小趋势,假手术组T2WI未见明显异常信号；1 H-MRS检测结果表明,针刺3d亚组和针刺7d亚组N-乙酰天门冬氨酸(N-acetylaspartate,NAA)较模型组明显升高(31.43±2.88)比(25.90±3.48,60.84±2.76比54.31±1.40；P均＜0.05),乳酸(lactate,Lac)明显降低(58.82±3.78比65.15±3.58,50.22±4.33比55.46±4.06；P均＜0.05),而胆碱复合物(choline,Cho)、肌酸(creatine,Cr)差异无统计学意义(P＞0.05).结论:1H-MRS检查能够客观评价针刺处理对MCAO大鼠梗死灶的干预作用,是研究脑梗死针刺治疗的可靠影像学方法.