小鼠肾脏急性缺血再灌注损伤不同时间点基因表达谱的差异

摘要

Objective:To analyze the stage‐specific differences in gene expression of mouse kidneys insulted by ischemia reper‐fusion(IR) injury .Methods:Mouse kidney tissues were collected at 4 h and 24 h after IR .Total RNA of control and IR kidneys were prepared for Microarray and real‐time fluorescence quantitative reverse transcription polymerase chain reaction(RT‐qPCR) analysis .Results:The number of differential expressed genes(DEGs) at 24 h after IR was larger than that at 4 h .The DEGs at 4 h were associated with metabolism and stress responses ,while the DEGs at 24 h were associated with inflammation and apop‐tosis .The numbers of up‐regulated genes at both 4 h and 24 h were larger than those of down‐regulated genes .Several down‐regulated ,up‐regulated ,or unchanged genes were confirmed by RT‐qPCR .Conclusions:Stage‐specific changes of gene expres‐sion occurres during IR injury .These DEGs at early or intermediate‐late stages of IR injury are tightly associated with their stage‐specific pathophysiological processes .%目的：探讨小鼠肾脏急性缺血再灌注损伤不同时间点基因表达谱的差异。方法：建立小鼠肾脏急性缺血再灌注损伤模型，收集再灌注4 h与24 h时缺血侧与对照侧肾脏组织标本，采用基因表达谱芯片技术检测各组标本的基因表达水平，再结合生物信息学技术分析差异表达的基因种类（gene ontology ，GO ）与信号通路，采用实时荧光定量逆转录聚合酶链反应法验证表达上调、下调与未变化的基因。结果：小鼠肾脏急性缺血再灌注24h差异表达的基因数目多于再灌注4h；再灌注4h时的变化基因主要为代谢与应激相关类型，24 h时的变化基因涉及炎性反应与细胞凋亡等；再灌注4 h和24 h时，缺血再灌注引起的表达上调的基因数目均多于表达下调的基因数目；随机挑选的表达上调、下调与未变化基因可以被实时荧光定量逆转录聚合酶链反应法验证。结论：缺血再灌注损伤伴随着大量基因表达的改变，缺血再灌注损伤不同时期的差异表达基因与病理生理变化密切相关。