2型糖尿病患者外周血中膜型及可溶性 CD28分子的表达及意义

摘要

目的：分析2型糖尿病患者外周血中膜型及可溶性 CD28分子的表达及其意义。方法分别收集2012年12月至2013年12月在北京军区联勤部门诊部就诊的30例2型糖尿病患者（糖尿病组）及同期30名健康体检者（对照组）的外周血，通过溶血及流式细胞术检测其外周血 T 细胞表面 CD28分子的表达，酶联免疫吸附（ELISA）法检测血清中可溶性 CD28的水平，并进行对比分析。结果糖尿病组外周血 CD4＋CD28＋T 细胞和 CD8＋CD28＋T 细胞显著低于对照组[（28．29±7．71）％比（32．77±4．28）％和（12．18±3．17）％比（18．53±3．27）％，均 P ＜0．01]，血清中可溶性 CD28水平显著高于对照组[（2．86±0．96）比（0．61±0．19）μg/L，P ＜0．01]。结论协同刺激分子 CD28很可能参与了2型糖尿病的免疫病理机制。%Objective To analyze the expression of membrane type CD28 and soluble CD28 in peripheral blood of type 2 diabetes patients.Methods The peripheral blood mononuclear cells (PBMCs)of 30 subjects with type 2 diabetes (diabetes group)and 30 healthy donors (control group)were collected from December 201 2 to De-cember 201 3 in the out -patient department of Outreach Logistics Department of Beijing Military Region.The expres-sion of CD28 on T cells in peripheral blood of type 2 diabetes patients was detected by flow cytometry (FCM).The secretion of soluble CD28 in serum was detected using the skills of Enzyme -linked immunosorbent assay (ELISA). The data was analyzed by the Graphpad Prism 5.01 program.Results The percentage of CD4 +CD28 +and CD8+CD28 +T cells in peripheral blood of type 2 diabetes patients were (28.29 ±7.71 )% and (1 2.1 8 ±3.1 7)% re-spectively,and were decreased markedly compared with healthy control (32.77 ±4.28% and 1 8.53 ±3.27%,P <0.01 and P <0.001 ).Compared with control,the level of soluble CD28 was increased significantly in serum of type 2 diabetes patients ((2.86 ±0.96)vs (0.61 ±0.1 9)μg/L,P <0.001 ).Conclusion The costimulatory mole-cule CD28 maybe take part in the immunological pathogenesis of type 2 diabetes.