川芎嗪对脓毒症大鼠肝细胞线粒体体视学的影响

摘要

Objective To study the protective effects and plausible mechanisms of tetramethylpyrazine (TMP)on the structure of hepatocyte mitochondria in septic rats.Methods The rats were randomly divided into four groups:control group,septic group,therapeutic group and prevention group.The model of septic rat was established by the cecal ligation and puncture(CLP).The liver was taken out from septic rat 10 h after CLP,.The ultrastructure of hepatocyte were observed by the JEM-1230 transmission electron microscope.The hepatocyte mitochondria were meas-ured by Image-Pro Plus 6.0 analysis software.The expression of AQP8 was assayed by Western blot.Results Com-pared with the control group,the total number of mitochondria and numberical density were fewer in other 3 groups. The total number of mitochondria and numberical density of septic group were more than the therapeutic and preven-tion group(P <0.01),but the volume density of septic group was lower than the control group(P <0.01).Compared with the control group,the relative AQP8 protein expression was significantly reduced in the other 3 groups(P <0.01).The AQP8 protein expression of the therapeutic and prevention group were higher than the septic group(P <0.01).Conclusion TMP can protect stereology of hepatocyte mitochondria in septicrats by increasing the expression of AQP8 protein.%目的：研究川芎嗪（TMP）对脓毒症大鼠肝细胞线粒体结构的保护作用及可能机制。方法实验大鼠被分成对照组、脓毒症组、治疗组和预防组；用盲肠结扎穿孔法制备大鼠脓毒症实验模型，在术后10 h，取肝脏标本用 JEM-1230透射电子显微镜观察肝细胞的超微结构，选用 Image-Pro Plus 6．0图像软件对线粒体进行测量；用 Western blot 技术检测肝细胞线粒体内膜水通道蛋白（AQP）8的表达。结果和对照组比较，其余3组单个肝细胞切面的线粒体总数和数密度均减少（P ＜0．01），但治疗组和预防组均多于脓毒症组（P ＜0．01）；线粒体体积密度脓毒症组也较对照组低（P ＜0．01）；线粒体内膜 AQP8的相对表达量在脓毒症组、治疗组和预防组均较对照组明显减少（P ＜0．01）；治疗组和预防组的 AQP8相对表达量较脓毒症组增多（P ＜0．01）。结论在脓毒症状态下，TMP 可保护肝细胞线粒体的结构，可能是通过增加线粒体内膜 AQP8表达而起作用的。