MicroRNA-133a在5-氟尿嘧啶促进胃癌细胞BGC823及SGC7901细胞凋亡中的功能研究

摘要

[目的]研究mieroRNA-133a(miR-133a)在5-氟尿嘧啶(5-Fu)诱导胃癌细胞BGC823及SGC7901凋亡中的作用.[方法]用荧光定量PCR检测经5-Fu(IC50值)处理过胃癌细胞miR-133a的表达;构建过表达miR-133a的胃癌细胞株并行细胞增殖、细胞流式验证miR-133a的生物学功能;5-Fu分别处理过表达及敲低miR-133a的胃癌细胞株后,行细胞增殖、细胞流式、Western Blot观察细胞生长及凋亡情况.[结果]5-Fu诱导BGC823及SGC7901内源性miR-133a的表达(＞50％,P＜0.0001,24 h),miR-133a促进胃癌细胞凋亡,敲低miR-133a可以抑制5-Fu对胃癌细胞的促凋亡作用.[结论]5-Fu通过诱导胃癌细胞BGC823及SGC7901内源性miR-133a的表达,促进胃癌细胞凋亡.%[Objective]To study the effect of microRNA-133a(miR-133a)on 5-fluorouracil(5-Fu)-induced apoptosis of gastric cancer BGC823 and SGC7901 cells.[Methods]The expression of miR-133a in gastric cancer cells treated with 5 Fu was detected by polymerase chain reaction(PCR);the biological function of miR-133a was confirmed by cell proliferation and cell flow assay.Over expression and knockdown of miR-133a gastric cancer cell lines were respectively tread with 5-Fu,and then cell proliferation,cell flow,Western Blot were designed to observe the growth and apoptosis of them.[Results]5-Fu induced BGC823 and SGC7901 endogenous miR-133a expression(＞50％,P＜0.0001,24 h);miR-133a could promote the apoptosis of gastric cancer cells;knockdown of miR-133a could inhibit the proapoptotic effect of 5-Fu on gastric cancer cells.[Conclusion]5-Fu promotes the apoptosis of gastric cancer cells by inducing the expression of endogenous miR-133a in BGC823 and SGC7901 cells.